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Purpose <p>The development of liposomal formulations for topical wound/burn treatment represents a promising therapeutic strategy. This study aimed to develop and characterize a nanoliposomal PPP formulation (PPP-Lip) to enhance drug delivery to wound sites while evaluating its stability, safety, and therapeutic efficacy.</p> Methods <p>PPP-Lip was prepared and characterized for size (171 ± 3.8 nm), zeta potential (-20 mV), and encapsulation efficiency (96%). Stability was assessed under accelerated (30 ℃/60% humidity for 6 months) and refrigerated (4 ℃ for 18 months) conditions using SDS-PAGE and circular dichroism. Safety was evaluated via MTT assay in HaCaT and HFFF-2 cells. Therapeutic potential was investigated through: 1) scratch wound assays measuring keratinocyte migration, 2) Franz cell diffusion studies assessing skin penetration, and 3) in vivo studies using BALB/c mouse wound/burn models.</p> Results <p>PPP-Lip demonstrated exceptional stability with no structural degradation observed. The formulation showed excellent biocompatibility (MTT assays) and significantly enhanced keratinocyte migration in wound healing assays. Franz cell studies revealed superior skin penetration of encapsulated PPP versus free PPP. In vivo results showed topical PPP-Lip application markedly accelerated wound/burn closure, reducing lesion diameter faster than controls.</p> Conclusion <p>PPP-Lip exhibits optimal physicochemical characteristics, long-term stability, and proven efficacy in both cellular and animal models. These findings position PPP-Lip as a highly promising topical therapeutic for wound/burn management, warranting further clinical investigation.</p>

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Development of nano-liposomal platelet-poor plasma (PPP) for topical application in wound healing and burn treatment

  • 期刊论文

  • 发表日期：

    2025年6月10日

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  • Reza Moradi,
  • Bita Taghizadeh,
  • Reza Ghaffari,
  • Farshad Mirzavi,
  • Bashir Sobhani,
  • Jebraeel Movaffagh,
  • Jalil Tavakol Afshari,
  • Hossein Kamali,
  • Jamshidkhan Chamani,
  • Seyedeh Hoda Alavizadeh,
  • Leila Arabi,
  • Marzieh Mohammadi,
  • Mahmoud Reza Jaafari

摘要

Purpose

The development of liposomal formulations for topical wound/burn treatment represents a promising therapeutic strategy. This study aimed to develop and characterize a nanoliposomal PPP formulation (PPP-Lip) to enhance drug delivery to wound sites while evaluating its stability, safety, and therapeutic efficacy.

Methods

PPP-Lip was prepared and characterized for size (171 ± 3.8 nm), zeta potential (-20 mV), and encapsulation efficiency (96%). Stability was assessed under accelerated (30 ℃/60% humidity for 6 months) and refrigerated (4 ℃ for 18 months) conditions using SDS-PAGE and circular dichroism. Safety was evaluated via MTT assay in HaCaT and HFFF-2 cells. Therapeutic potential was investigated through: 1) scratch wound assays measuring keratinocyte migration, 2) Franz cell diffusion studies assessing skin penetration, and 3) in vivo studies using BALB/c mouse wound/burn models.

Results

PPP-Lip demonstrated exceptional stability with no structural degradation observed. The formulation showed excellent biocompatibility (MTT assays) and significantly enhanced keratinocyte migration in wound healing assays. Franz cell studies revealed superior skin penetration of encapsulated PPP versus free PPP. In vivo results showed topical PPP-Lip application markedly accelerated wound/burn closure, reducing lesion diameter faster than controls.

Conclusion

PPP-Lip exhibits optimal physicochemical characteristics, long-term stability, and proven efficacy in both cellular and animal models. These findings position PPP-Lip as a highly promising topical therapeutic for wound/burn management, warranting further clinical investigation.