<p>Soil carbon sequestration represents a crucial methodology for attenuating the effects of climate change; however, the precise measurement of soil organic carbon (SOC) reservoirs along with their interactions with nitrogen and phosphorus presents a formidable challenge.&#xa0;This investigation aims to bridge this gap by utilizing the cool farm tool (CFT), an instrument based on the IPCC guideline, to evaluate SOC dynamics, the capacity for carbon sequestration, and nutrient interactions within sugarcane agricultural systems. Centered on 15 distinct sites, we gathered input data via interviews with farmers and conducted analyses of 75 soil samples to ascertain levels of SOC, pH, nitrogen, and phosphate. The findings indicated that sites characterized by low SOC levels demonstrated the greatest potential for future carbon sequestration, with total nitrogen concentrations fluctuating between 2.04 and 9.56 kg-t and phosphate concentrations ranging from 300.33 to 411.39 kg. The overall subsurface carbon stock was approximated at 1,801,180 tons of CO₂, highlighting the substantial carbon sequestration potential inherent in these agricultural lands. This research illustrates that the CFT serves as a reliable and effective instrument for measuring carbon sequestration and nutrient dynamics within agricultural contexts, exhibiting minimal uncertainties and data deficiencies. By pinpointing areas with elevated carbon efficiency and saturation, this study furnishes pragmatic insights that can be leveraged to refine agricultural practices aimed at augmenting SOC storage and addressing climate change.</p>