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Objectives <p>Vascular graft/endograft infections (VGEI) are difficult-to-treat and associated with high morbidity and mortality. Up to 40% of VGEI are polymicrobial, complicating clinical management. This study investigates microbial interactions and their impact on clinical outcomes.</p> Methods <p>We analyzed patient characteristics and survival in poly- versus monomicrobial infections from the VASGRA cohort, a Swiss prospective observational study. Kaplan–Meier analysis and Cox proportional hazards models were used to assess survival. Furthermore, a subset of patient-derived bacterial strains underwent Quantitative Fitness Analysis (QFA) to quantify changes in fitness in mono- and polymicrobial conditions.</p> Results <p>Among 227 VGEI episodes, 81 (35.7%) were polymicrobial. Overall survival was 87.7% at one year and 77.5% at five years, with polymicrobial infections associated with reduced survival (log-rank <i>p</i> = 0.004). Multivariable analyses confirmed that polymicrobial infections (adjusted HR = 2.94, 95% CI: 1.60–5.41, <i>p</i> &lt; 0.001) and age per 10&#xa0;years older (adjusted HR = 2.07, 95% CI: 1.26–3.41, <i>p</i> = 0.004) were independent risk factors for increased mortality. There was also a trend of an association with diabetes mellitus (adjusted HR = 1.95, 95% CI: 0.91–4.29, <i>p</i> = 0.088). QFA of five strain pairs revealed diverse interaction patterns among pathogens, ranging from complete inhibition to enhanced growth in co-culture.</p> Conclusion <p>Polymicrobial infections, along with older age and diabetes mellitus, were identified as independent risk factors for increased mortality in VGEI patients. QFA revealed diverse bacterial interactions, highlighting the complexity of polymicrobial environments in VGEI.</p>

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Clinical and microbial insights into polymicrobial vascular graft infections: a prospective observational study

  • 期刊论文

  • 发表日期：

    2026年3月26日

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  • Catrina I. Pedrett,
  • Jana Epprecht,
  • Bruno Ledergerber,
  • Julian Bär,
  • Judith Bergada-Pijuan,
  • Claudio T. Acevedo,
  • Andrea Tarnutzer,
  • Benedikt Reutersberg,
  • Pascal M. Frey,
  • Annelies S. Zinkernagel,
  • Silvio D. Brugger,
  • Barbara Hasse

摘要

Objectives

Vascular graft/endograft infections (VGEI) are difficult-to-treat and associated with high morbidity and mortality. Up to 40% of VGEI are polymicrobial, complicating clinical management. This study investigates microbial interactions and their impact on clinical outcomes.

Methods

We analyzed patient characteristics and survival in poly- versus monomicrobial infections from the VASGRA cohort, a Swiss prospective observational study. Kaplan–Meier analysis and Cox proportional hazards models were used to assess survival. Furthermore, a subset of patient-derived bacterial strains underwent Quantitative Fitness Analysis (QFA) to quantify changes in fitness in mono- and polymicrobial conditions.

Results

Among 227 VGEI episodes, 81 (35.7%) were polymicrobial. Overall survival was 87.7% at one year and 77.5% at five years, with polymicrobial infections associated with reduced survival (log-rank p = 0.004). Multivariable analyses confirmed that polymicrobial infections (adjusted HR = 2.94, 95% CI: 1.60–5.41, p < 0.001) and age per 10 years older (adjusted HR = 2.07, 95% CI: 1.26–3.41, p = 0.004) were independent risk factors for increased mortality. There was also a trend of an association with diabetes mellitus (adjusted HR = 1.95, 95% CI: 0.91–4.29, p = 0.088). QFA of five strain pairs revealed diverse interaction patterns among pathogens, ranging from complete inhibition to enhanced growth in co-culture.

Conclusion

Polymicrobial infections, along with older age and diabetes mellitus, were identified as independent risk factors for increased mortality in VGEI patients. QFA revealed diverse bacterial interactions, highlighting the complexity of polymicrobial environments in VGEI.