Scholarlink

<Emphasis Type="BoldItalic">BACKGROUND: </Emphasis> <p>Mesenchymal stem cells (MSCs) are multipotent mesoderm-derived cells with high proliferation capacity, self-renewal, paracrine activity, and multilineage differentiation potential. Autologous MSCs from the same patients are particularly valuable for regenerative therapies, including those targeting degenerative intervertebral disc disorder (DIVDD).</p> <Emphasis Type="BoldItalic">METHODS: </Emphasis> <p>In this study, we directly compared autologous MSC sources obtained from the same patients, thereby reducing biological variability and strengthening translational relevance. We compared MSCs isolated from bone marrow (BM–MSCs), adipose tissue (AD–MSCs), and nucleus pulposus (NP–MSCs) obtained from the same DIVDD patient donor (<i>N</i> = 10 donors). We assessed their morphologies, proliferation abilities, immunophenotype, multilineage differentiation capacity, and chondrogenic differentiation–related gene and protein expression.</p> <Emphasis Type="BoldItalic">RESULTS: </Emphasis> <p>All three MSC populations displayed similar fibroblast-like morphology and consistent immunophenotypic profiles. However, their functional properties differed: AD–MSCs exhibited significantly higher proliferative activity and greater adipogenic differentiation potential (<i>p</i> &lt; 0.01 and <i>p</i> &lt; 0.001), while osteogenic and chondrogenic differentiation capacities were comparable across BM-MSCs, AD-MSCs, and NP-MSCs at both gene and protein levels (SOX9 and COMP).</p> <Emphasis Type="BoldItalic">CONCLUSION: </Emphasis> <p>These findings suggest that AD-MSCs offer practical advantages in terms of proliferation capacity and accessibility. However, chondrogenic differentiation capacity was largely comparable across MSC sources. Further mechanistic studies and <i>in vivo</i> validation are required to determine their relative therapeutic efficacy for intervertebral disc regeneration.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Comparative Study of Mesenchymal Stem Cell Identity and Chondrogenic Differentiation Capacity of Human Nucleus Pulposus, Adipose and Bone Marrow Tissues Derived Mesenchymal Stem Cells

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年7月22日

查看全文
  • Hataiwan Kunkanjanawan,
  • Tanut Kunkanjanawan,
  • Veerapol Khemarangsan,
  • Siravich Suvithayasiri,
  • Tawit Suriyo,
  • Jutamaad Satayavivad,
  • Warayos Trathitephun

摘要

BACKGROUND:

Mesenchymal stem cells (MSCs) are multipotent mesoderm-derived cells with high proliferation capacity, self-renewal, paracrine activity, and multilineage differentiation potential. Autologous MSCs from the same patients are particularly valuable for regenerative therapies, including those targeting degenerative intervertebral disc disorder (DIVDD).

METHODS:

In this study, we directly compared autologous MSC sources obtained from the same patients, thereby reducing biological variability and strengthening translational relevance. We compared MSCs isolated from bone marrow (BM–MSCs), adipose tissue (AD–MSCs), and nucleus pulposus (NP–MSCs) obtained from the same DIVDD patient donor (N = 10 donors). We assessed their morphologies, proliferation abilities, immunophenotype, multilineage differentiation capacity, and chondrogenic differentiation–related gene and protein expression.

RESULTS:

All three MSC populations displayed similar fibroblast-like morphology and consistent immunophenotypic profiles. However, their functional properties differed: AD–MSCs exhibited significantly higher proliferative activity and greater adipogenic differentiation potential (p < 0.01 and p < 0.001), while osteogenic and chondrogenic differentiation capacities were comparable across BM-MSCs, AD-MSCs, and NP-MSCs at both gene and protein levels (SOX9 and COMP).

CONCLUSION:

These findings suggest that AD-MSCs offer practical advantages in terms of proliferation capacity and accessibility. However, chondrogenic differentiation capacity was largely comparable across MSC sources. Further mechanistic studies and in vivo validation are required to determine their relative therapeutic efficacy for intervertebral disc regeneration.