Scholarlink

Background <p>Regenerative cell sources such as bone marrow-multipotent stem cells (BM-MSCs) face poor survival after transplantation due to shear stress and anoikis in attachment-deprived conditions. Cell surface modification with type I collagen (Col I) may enhance cell survival in anoikis-inducing environment by mimicking cell-ECM interactions and activating Akt signaling. However, the relative contributions of biochemical versus mechanical signalling remain unclear.</p> Methods <p>BM-MSCs were surface-modified with 2, 4, or 8 layers (L) of Col I and cultured on poly-2-hydroxyethyl methacrylate-coated vessels for 3&#xa0;days. Col I retention, Akt activation, and cytoskeletal changes were analyzed by immunofluorescence. YAP nuclear translocation was measured to assess mechanotransduction.</p> Results <p>Col I persisted up to 10&#xa0;h in 4L and 8L groups but not in 2L. All surface-modified groups showed membrane-localized Akt phosphorylation, while controls did not. Only the 8L group demonstrated significant anoikis resistance from day 1, exhibiting distinct ring-like actin arrangement within 2&#xa0;h and enhanced YAP nuclear localization, indicating activation of mechanotransduction-associated responses.</p> Conclusions <p>All surface-modified groups showed Akt phosphorylation, indicating biochemical signaling. The 4L group exhibited significantly higher survival by day 2, suggesting sustained biochemical signaling promotes survival. The 8L group showed superior survival from day 1 with increased YAP translocation and actin reorganization, demonstrating that mechanotransduction may contribute to an early survival advantage. This cell model enables differential investigation of biochemical and mechanical effects on cell survival.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Biochemical and Mechanical Signal Differentially Contribute to Survival in Surface-Modified Cell Model

  • 期刊论文

  • 发表日期：

    2026年3月14日

查看全文
  • Seoyoung Jang,
  • Tong In Oh,
  • Wook Park,
  • EunAh Lee

摘要

Background

Regenerative cell sources such as bone marrow-multipotent stem cells (BM-MSCs) face poor survival after transplantation due to shear stress and anoikis in attachment-deprived conditions. Cell surface modification with type I collagen (Col I) may enhance cell survival in anoikis-inducing environment by mimicking cell-ECM interactions and activating Akt signaling. However, the relative contributions of biochemical versus mechanical signalling remain unclear.

Methods

BM-MSCs were surface-modified with 2, 4, or 8 layers (L) of Col I and cultured on poly-2-hydroxyethyl methacrylate-coated vessels for 3 days. Col I retention, Akt activation, and cytoskeletal changes were analyzed by immunofluorescence. YAP nuclear translocation was measured to assess mechanotransduction.

Results

Col I persisted up to 10 h in 4L and 8L groups but not in 2L. All surface-modified groups showed membrane-localized Akt phosphorylation, while controls did not. Only the 8L group demonstrated significant anoikis resistance from day 1, exhibiting distinct ring-like actin arrangement within 2 h and enhanced YAP nuclear localization, indicating activation of mechanotransduction-associated responses.

Conclusions

All surface-modified groups showed Akt phosphorylation, indicating biochemical signaling. The 4L group exhibited significantly higher survival by day 2, suggesting sustained biochemical signaling promotes survival. The 8L group showed superior survival from day 1 with increased YAP translocation and actin reorganization, demonstrating that mechanotransduction may contribute to an early survival advantage. This cell model enables differential investigation of biochemical and mechanical effects on cell survival.