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<Emphasis Type="BoldItalic">BACKGROUND</Emphasis> <p>Xenogeneic menisci offer a promising biomaterial with high biocompatibility, closely resembling the native meniscus. However, to enable clinical application in humans, decellularization is essential to remove cellular components that may trigger graft rejection.</p> <Emphasis Type="BoldItalic">METHOD</Emphasis> <p>We developed a combined decellularization protocol for bovine-derived meniscus tissue, integrating physical stimulation (sonication and vacuum), chemical treatments (hypotonic and hypertonic solutions, and sodium dodecyl sulfate [SDS]), and enzymatic digestion (trypsin and nucleases). The decellularization efficiency was confirmed not only by DNA reduction rate, but also by residual DNA, DNA fragmentation and histology. Subsequently, cytotoxicity, biocompatibility, and mechanical properties were assessed.</p> <Emphasis Type="BoldItalic">RESULTS</Emphasis> <p>This combined decellularization achieved a DNA removal efficiency of up to 94.94%, enabled rapid decellularization within 5&#xa0;days, and preserved collagen content, resulting in a high-quality xeno meniscal implant (XMI).</p> <Emphasis Type="BoldItalic">CONCLUSION</Emphasis> <p>The XMI produced through this combined decellularization process demonstrates strong potential as a scaffold for the treatment of meniscal injuries.</p>

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Rapid and Efficient Combined Decellularization with Physical, Chemical and Enzymatic Treatment for Production of Bovine-Derived Meniscal Implant to Replace Damaged Meniscus

  • 期刊论文

  • 发表日期：

    2026年3月13日

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  • Young-Bock Shim,
  • Sang Hoon Lee,
  • YoungWoo Ryu,
  • Sang hyun Jung,
  • Shin Young Park,
  • Yu jin Lee,
  • Hong Hee Jung,
  • Dae Hyeok Yang,
  • Heung Jae Chun

摘要

BACKGROUND

Xenogeneic menisci offer a promising biomaterial with high biocompatibility, closely resembling the native meniscus. However, to enable clinical application in humans, decellularization is essential to remove cellular components that may trigger graft rejection.

METHOD

We developed a combined decellularization protocol for bovine-derived meniscus tissue, integrating physical stimulation (sonication and vacuum), chemical treatments (hypotonic and hypertonic solutions, and sodium dodecyl sulfate [SDS]), and enzymatic digestion (trypsin and nucleases). The decellularization efficiency was confirmed not only by DNA reduction rate, but also by residual DNA, DNA fragmentation and histology. Subsequently, cytotoxicity, biocompatibility, and mechanical properties were assessed.

RESULTS

This combined decellularization achieved a DNA removal efficiency of up to 94.94%, enabled rapid decellularization within 5 days, and preserved collagen content, resulting in a high-quality xeno meniscal implant (XMI).

CONCLUSION

The XMI produced through this combined decellularization process demonstrates strong potential as a scaffold for the treatment of meniscal injuries.