<p>The extensive use and improper disposal of antibiotics have emerged as major environmental concerns, placing them among the most prominent contaminants on the list of emerging pollutants. Antibiotic pollution has become a widespread environmental problem that requires a holistic approach to curb this. In the current study, three antibiotics—Ciprofloxacin (CIP), Amoxicillin (AMX), and Tetracycline (TET)—were targeted for monitoring, owing to their longer half-life, high pollution levels, and importance in healthcare. Their untamed spread in reservoirs of our ecosystem has directed the spread of Antibiotic Resistance (AbR). Molecularly Imprinted Polymers (MIPs), being synthetic and custom-tailored for specific targets; offer high specificity and stability for selective detection of antibiotics. The study explores the use of single unified methodology for the synthesis of MIPs against three antibiotics with an aim to curb the limitations of conventional methods yet providing an eay-to-use prototype for specific detection of antibiotics. Quantum mechanical DFT calculations were employed to understand the chemical interaction between template and monomer. Moreover, the use of SPIONs (γ-Fe₂O₃ nanoparticles) as core facilitates the efficient retrieval of MIPs from the bulk samples and, hence, the antibiotics. Mag-MIPs were extensively characterized via SEM, TEM, EDX, FT-IR, XRD and TGA-DSC. The sorption experiments showed that Mag-MIPs unanimously followed Langmuir adsorption isotherm and pseudo-second-order kinetic models. They possess excellent reusability for upto three cycles of adsorption/desorption. Binding tests with similar compounds shows excellent selectivity of Mag-MIPs for the target antibiotic. Limit of Detection (Mag-MIP CIP (0.03 μg/L), Mag-MIP AMX (0.02 μg/L), Mag-MIP TET (0.02 μg/L)) for the developed prototype were well within the predicted no-effect concentration for resistance (PNEC^R).</p>