<p>During the past decade, electrokinetic remediation and its associated hybrid technologies have emerged as new technologies for removal of organic pollutants from contaminated soils. To address hydrocarbon-contaminated soils, a novel myco-electrochemical remediation approach was designed and evaluated within the current study. For the first time, the coupling of electrochemical with myco-augmentation, using <i>Pleurotus ostreatus</i> Spent Mushroom Substrate as inoculum to degrade Total Petroleum Hydrocarbons from soil was applied as part of an experimental study. Furthermore, myco-augmentation and single electrokinetic treatment were assessed in relation to two distinct hydrocarbon-contaminated soils: an area with oil and natural gas extraction wells (clay soil), and agricultural land (sandy soil) where an unintentional spill occurred. Single treatments have been also evaluated separately for both soil texture. The experimental outcomes revealed that Mycoremediation tends to perform better in soils with a sandy texture (removal rates reached 37.15% in sandy soil, compared to 34.55% in clay soil). The trend was reversed when the electrochemical method was employed. Electrokinetic treatment worked better for clay soils, with a removal rate of 45.69% for, compared to 14.57% for clay soil. Electroremediation and Mycoremediation alone were not as effective as the combined application of the two distinct remediation technologies. With the sequential approach, abatement rates of 48.63% for sandy soil and 53.44% for clay soil were achieved. Resulted in this way that, integrating electrokinetic and mycoremediation techniques offers a sustainable strategy for remediating soils contaminated with petroleum hydrocarbons, while reducing the overall energy consumption and costs in soil remediation.</p>