<p><i>Alphitobius diaperinus</i> Panzer, 1797 (Coleoptera: Tenebrionidae), a common insect in poultry farms, poses a significant threat to poultry production. However, this insect also shows potential as a nutritional source due to its high protein and fat content. Studying its biological cycle and metabolic profile is essential for understanding its biology and biochemistry, enabling the development of more effective control strategies based on biochemical targets. This study aimed to investigate the biological and metabolic aspects of <i>A. diaperinus</i> at different developmental stages, providing relevant information for future research. Insect samples, maintained in colonies, were analyzed morphologically and metabolically. Morphological measurements were performed using digitized images, while metabolic profiles were obtained through gas chromatography coupled with mass spectrometry. The average biological cycle was 54&#xa0;days, from egg to adult. Morphological parameters revealed distinct patterns of larval growth, with a positive correlation between length and age. Significant biochemical transformations were observed in the levels of amino acids, sugars, and organic acids throughout development, reflecting the specific metabolic needs of each stage. These findings expand the knowledge of the biological and biochemical aspects of <i>A. diaperinus</i>, providing a foundation for more effective and sustainable management strategies for controlling this pest.</p> Graphical Abstract <p></p>