Green synthesis of immobilized Ag NPs on the magnetic agar (Fe₃O₄@Agar-Ag NPs) as reusable nanobiocatalyst and its inhibitory effect on the MCF-7 cancerous cell lines

The magnetic biocompatible Fe₃O₄@Agar-Ag catalyst was designed and prepared based on a natural macromolecule (agar) through a convenient method using inexpensive, nontoxic, and easily available substances. Following synthesis, the catalyst underwent characterization using various techniques such as FT-IR, XRD, FE-SEM, EDX, VSM, TGA, and ICP. Subsequently, its efficacy was evaluated in treating breast cancer, in vitro. The results demonstrated the catalyst's effectiveness in reducing the proliferation of MCF-7 cells, suggesting its potential as an anticancer agent. Furthermore, our findings highlighted the biocompatibility of the synthesized nanoparticle and its lower toxicity toward normal cells.