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<p>The ameliorating property of biopolymer-encapsulated probiotic compared to non-encapsulated probiotic as an adjunct therapy in the treatment of chronic kidney disease (CKD) in experimental rat model was evaluated in this study. <i>Lactobacillus acidophilus</i> strain designation NCIMB 1899 [NCDO 2], ATCC 11975 strain was obtained in freeze dried form from National Collection of Dairy Cultures (NCDC). Alginate and pectin biopolymer-encapsulated <i>L. acidophilus</i> microcapsules were produced with 60% encapsulation efficiency, and excellent viability in low pH, colonic pH, and high bile salt concentration. The microsphere gel particles with diameters of 0.8 to 2.5&#xa0;mm were obtained from modified extrusion technique. Scanning electron microscopy revealed spherical beads with slightly rugged surface. CKD was established in a rat model by 28th day of induction with two doses of intraperitoneal folic acid injection 2&#xa0;weeks apart at 250&#xa0;mg/kg body weight, which was confirmed by hematobiochemical values and histopathological alterations in kidneys. The kidney sections revealed extensive damage to glomeruli, along with necrosis and calcification of the tubular lining in rats treated with folic acid. Increased peri-tubular and peri-glomerular cellular reactions were observed throughout the tissue. Encapsulated probiotic dosed at 1.0 × 10<sup>8–10</sup>&#xa0;cfu, twice a day, for 28&#xa0;days depicted better efficacy in ameliorating CKD than the non-encapsulated probiotic, which was confirmed by hematobiochemical, and histopathological evaluations, presumably due to increased bioavailability at the target tissue of the host.</p> Graphical abstract <p></p>

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Ameliorative potentials of alginate–pectin-encapsulated Lactobacillus acidophilus probiotics in experimental model of CKD in rats

  • 期刊论文

  • 发表日期：

    2025年8月20日

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  • Kaveri Jambagi,
  • Debabrata Mondal,
  • Raguvaran Raja,
  • Ensha Lomiya,
  • Shivangi Dosar,
  • Rajshekhar Kamalla,
  • K. S. Suhas,
  • Bharat Shindhe,
  • Akhilesh Kumar

摘要

The ameliorating property of biopolymer-encapsulated probiotic compared to non-encapsulated probiotic as an adjunct therapy in the treatment of chronic kidney disease (CKD) in experimental rat model was evaluated in this study. Lactobacillus acidophilus strain designation NCIMB 1899 [NCDO 2], ATCC 11975 strain was obtained in freeze dried form from National Collection of Dairy Cultures (NCDC). Alginate and pectin biopolymer-encapsulated L. acidophilus microcapsules were produced with 60% encapsulation efficiency, and excellent viability in low pH, colonic pH, and high bile salt concentration. The microsphere gel particles with diameters of 0.8 to 2.5 mm were obtained from modified extrusion technique. Scanning electron microscopy revealed spherical beads with slightly rugged surface. CKD was established in a rat model by 28th day of induction with two doses of intraperitoneal folic acid injection 2 weeks apart at 250 mg/kg body weight, which was confirmed by hematobiochemical values and histopathological alterations in kidneys. The kidney sections revealed extensive damage to glomeruli, along with necrosis and calcification of the tubular lining in rats treated with folic acid. Increased peri-tubular and peri-glomerular cellular reactions were observed throughout the tissue. Encapsulated probiotic dosed at 1.0 × 108–10 cfu, twice a day, for 28 days depicted better efficacy in ameliorating CKD than the non-encapsulated probiotic, which was confirmed by hematobiochemical, and histopathological evaluations, presumably due to increased bioavailability at the target tissue of the host.

Graphical abstract