<p>A chemical constituent study on the fermented rice substrate of basidiomycetous fungus <i>Panus rudis</i> led to the isolation of four previously undescribed prenylhydroquinone derivatives compounds (<b>1</b>–<b>4</b>) and eight known compounds (<b>5</b>–<b>12</b>). Among them, compound <b>3</b> featured a rare benzothiazole derivative with hydroxy substituted 3-methyl-1-butenyl substitution on the benzene ring, and the absolute configurations of <b>7</b> and <b>12</b> were elucidated as unreported ones. Their structures were identified by the interpretation of 1D and 2D NMR spectroscopy, HRESIMS data, X-ray single-crystal diffraction, and comparison of calculated and experimental ECD spectra. The plausible biosynthetic pathways for <b>1</b>–<b>7</b> are proposed. Cytotoxicity evaluation was conducted on <b>1</b> and <b>3</b>–<b>12</b> against two cancer cell lines (A-549 and HepG2). The results demonstrated that <b>1</b>, <b>3</b>–<b>6</b>, <b>8</b>, <b>9</b>, and <b>12</b> exhibited weak cytotoxicity against both two cell lines. Among them, <b>8</b> and <b>12</b> showed dose-dependent inhibitory effects, and their IC<sub>50</sub> values at 72 h were obtained.</p> Graphical Abstract <p></p>

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Panunoids A – D, four new prenylhydroquinone derivatives isolated from the fungus Panus rudis

  • Yun Liu,
  • Jian-Qiang Zhao,
  • Yan-Long Yang,
  • Han-Bing Yuan,
  • Yan-Ming Wang,
  • Jun Yuan

摘要

A chemical constituent study on the fermented rice substrate of basidiomycetous fungus Panus rudis led to the isolation of four previously undescribed prenylhydroquinone derivatives compounds (14) and eight known compounds (512). Among them, compound 3 featured a rare benzothiazole derivative with hydroxy substituted 3-methyl-1-butenyl substitution on the benzene ring, and the absolute configurations of 7 and 12 were elucidated as unreported ones. Their structures were identified by the interpretation of 1D and 2D NMR spectroscopy, HRESIMS data, X-ray single-crystal diffraction, and comparison of calculated and experimental ECD spectra. The plausible biosynthetic pathways for 17 are proposed. Cytotoxicity evaluation was conducted on 1 and 312 against two cancer cell lines (A-549 and HepG2). The results demonstrated that 1, 36, 8, 9, and 12 exhibited weak cytotoxicity against both two cell lines. Among them, 8 and 12 showed dose-dependent inhibitory effects, and their IC50 values at 72 h were obtained.

Graphical Abstract