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<p><i>Cotinus coggygria</i> Scop. is traditionally used for its wound-healing, antimicrobial, and anti-inflammatory properties. Many phenolic and flavonoid compounds isolated from this plant have been extensively documented for their anticancer properties; however, data on the antitumor potential of the crude extract and the mechanisms underlying its effects are scarce. The aim of the study was to investigate the antitumor effects of <i>C. coggygria</i> Scop. leaf and bark crude methanol extracts on tumor cell lines. Chemical analysis was performed using HPLC. The cytotoxic effect of extracts against HCT116 and HeLa tumor cell lines and non-transformed MRC-5 cells was estimated using the MTT assay. The type of cell death and the impact on cell cycle progression were assessed by flow cytometry. Wound healing assay was employed for cell migration studies. The nature of interactions with doxorubicin was analysed with the SinergyFinder+ platform. Both extracts exhibited moderate to pronounced and notably selective cytotoxic effects against tumor cells. Extracts induced apoptosis, as evidenced by the change in the Bax/Bcl-2 ratio, mitochondrial damage, activation of caspase-3, and the disruption of cell cycle progression. Both extracts potentiated the cytotoxic effects of doxorubicin and markedly suppressed the migratory capacity of HCT116 and HeLa cells in vitro. The results of the study indicate the potential of <i>C. coggygria</i> Scop. leaf and bark crude extracts as complementary agents in cancer therapy.</p>

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Beyond traditional medicine: Cotinus coggygria Scop. extracts as promising adjuvants in cancer therapy

  • 期刊论文

  • 发表日期：

    2026年3月7日

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  • Danijela Todorovic,
  • Suzana Popovic,
  • Vasilija Srdanovic,
  • Dejan Baskic,
  • Sanja Matic,
  • Tijana Markovic,
  • Milica Markovic,
  • Ana Todorovic,
  • Andrija Ciric,
  • Nenad Zlatic,
  • Milan Stankovic

摘要

Cotinus coggygria Scop. is traditionally used for its wound-healing, antimicrobial, and anti-inflammatory properties. Many phenolic and flavonoid compounds isolated from this plant have been extensively documented for their anticancer properties; however, data on the antitumor potential of the crude extract and the mechanisms underlying its effects are scarce. The aim of the study was to investigate the antitumor effects of C. coggygria Scop. leaf and bark crude methanol extracts on tumor cell lines. Chemical analysis was performed using HPLC. The cytotoxic effect of extracts against HCT116 and HeLa tumor cell lines and non-transformed MRC-5 cells was estimated using the MTT assay. The type of cell death and the impact on cell cycle progression were assessed by flow cytometry. Wound healing assay was employed for cell migration studies. The nature of interactions with doxorubicin was analysed with the SinergyFinder+ platform. Both extracts exhibited moderate to pronounced and notably selective cytotoxic effects against tumor cells. Extracts induced apoptosis, as evidenced by the change in the Bax/Bcl-2 ratio, mitochondrial damage, activation of caspase-3, and the disruption of cell cycle progression. Both extracts potentiated the cytotoxic effects of doxorubicin and markedly suppressed the migratory capacity of HCT116 and HeLa cells in vitro. The results of the study indicate the potential of C. coggygria Scop. leaf and bark crude extracts as complementary agents in cancer therapy.