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<p>Stromal interaction molecule 2(STIM2) has been implicated in the progression of various kinds of tumors. Existing researches have not explored its effect on how tumor-associated macrophages (TAMs) are polarized into the M2 phenotype in cholangiocarcinoma. CCA cells underwent co-culture with an M2-like conditioned medium (M2-CM). We established the subcutaneous xenograft model of nude mice. The western blot together with quantitative real-time PCR (qPCR) assisted in the determination of molecule expression. STIM2 suppresses anti-tumor immunity in CCA through inhibiting CD8 + T cell under the mediation of TAM. STIM2 induces TAMs to be transformed into pro-tumorigenic M2-like phenotypes through the activation of cAMP signaling pathway. STIM2 holds a large potential of serving for the immunotherapy targeting CCA by virtue of TAMs. The molecular mechanism regarding its tumor-promoting effect in CCA is elucidated in the study.</p>

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STIM2 suppressed Anti-tumor immunity in cholangiocarcinoma

  • 期刊论文

  • 发表日期：

    2025年10月28日

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  • Li-Ping Ying,
  • Zong-Yang Wu,
  • Jin-Bo Wang,
  • Ji-Ye Feng,
  • Kai Lu

摘要

Stromal interaction molecule 2(STIM2) has been implicated in the progression of various kinds of tumors. Existing researches have not explored its effect on how tumor-associated macrophages (TAMs) are polarized into the M2 phenotype in cholangiocarcinoma. CCA cells underwent co-culture with an M2-like conditioned medium (M2-CM). We established the subcutaneous xenograft model of nude mice. The western blot together with quantitative real-time PCR (qPCR) assisted in the determination of molecule expression. STIM2 suppresses anti-tumor immunity in CCA through inhibiting CD8 + T cell under the mediation of TAM. STIM2 induces TAMs to be transformed into pro-tumorigenic M2-like phenotypes through the activation of cAMP signaling pathway. STIM2 holds a large potential of serving for the immunotherapy targeting CCA by virtue of TAMs. The molecular mechanism regarding its tumor-promoting effect in CCA is elucidated in the study.