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<p>Plants have long been recognized for their therapeutic potential, with numerous species exhibiting diverse pharmacological properties, including hepatoprotective, antioxidant, and anti-inflammatory effects. <i>Cotinus coggygria</i>, a traditionally used medicinal plant, has demonstrated various biological activities, yet its hepatoprotective potential against drug-induced liver toxicity remains underexplored. This study aimed to evaluate the hepatoprotective effect of the methanolic extract of <i>Cotinus coggygria</i> in paracetamol-induced acute and subacute liver toxicity in rabbits, addressing a critical research gap in exploring natural substances as hepatoprotective agents. Biochemical and histopathological analyses were conducted to assess liver function and tissue integrity. In acute toxicity studies, pretreatment with 250&#xa0;mg and 500&#xa0;mg of the plant extract significantly ameliorated (<i>p</i> &lt; 0.05) paracetamol-induced hepatocyte damage, as evidenced by improved biochemical markers and histopathological findings. A lower dose (100&#xa0;mg and 200&#xa0;mg) exhibited significant hepatoprotective effects in the subacute hepatotoxicity model. To ensure the safety of prolonged use, in vitro cytotoxicity was evaluated using lower doses of the extract, e.g. 6.25&#xa0;µM to 800&#xa0;µM, using normal human hepatic cell lines THLE-3 and IHH, via MTT assay. The results confirmed no evidence of cytotoxic effects on healthy liver cells. These findings highlight the dose-dependent hepatoprotective efficacy of <i>Cotinus coggygria</i> methanolic extract, supported by robust methodological approaches and conclusive biochemical and histological evidence. The study concludes that <i>Cotinus coggygria</i> extract holds significant promise as a natural hepatoprotective agent against paracetamol-induced liver injury, warranting further clinical and molecular investigations to validate its optimized therapeutic potential.</p> Graphical abstract <p></p>

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Evaluation of the hepatoprotective activity of the methanolic extract of Cotinus coggygria

  • 期刊论文

  • 发表日期：

    2025年9月2日

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  • Shahzad Kamran,
  • Sairah Hafeez Kamran,
  • Mohsin Ahmad Ghauri,
  • Ali Raza,
  • Muhammad Ajaib

摘要

Plants have long been recognized for their therapeutic potential, with numerous species exhibiting diverse pharmacological properties, including hepatoprotective, antioxidant, and anti-inflammatory effects. Cotinus coggygria, a traditionally used medicinal plant, has demonstrated various biological activities, yet its hepatoprotective potential against drug-induced liver toxicity remains underexplored. This study aimed to evaluate the hepatoprotective effect of the methanolic extract of Cotinus coggygria in paracetamol-induced acute and subacute liver toxicity in rabbits, addressing a critical research gap in exploring natural substances as hepatoprotective agents. Biochemical and histopathological analyses were conducted to assess liver function and tissue integrity. In acute toxicity studies, pretreatment with 250 mg and 500 mg of the plant extract significantly ameliorated (p < 0.05) paracetamol-induced hepatocyte damage, as evidenced by improved biochemical markers and histopathological findings. A lower dose (100 mg and 200 mg) exhibited significant hepatoprotective effects in the subacute hepatotoxicity model. To ensure the safety of prolonged use, in vitro cytotoxicity was evaluated using lower doses of the extract, e.g. 6.25 µM to 800 µM, using normal human hepatic cell lines THLE-3 and IHH, via MTT assay. The results confirmed no evidence of cytotoxic effects on healthy liver cells. These findings highlight the dose-dependent hepatoprotective efficacy of Cotinus coggygria methanolic extract, supported by robust methodological approaches and conclusive biochemical and histological evidence. The study concludes that Cotinus coggygria extract holds significant promise as a natural hepatoprotective agent against paracetamol-induced liver injury, warranting further clinical and molecular investigations to validate its optimized therapeutic potential.

Graphical abstract