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<p>Samul-tang (SMT) is a well-known traditional herbal medicine. Recent studies have reported that SMT exhibits protective biological effects against inflammation, oxidative stress, and cancer. It is commonly used to treat gynecological disorders, including irregular menstruation, pre-eclampsia, and postmenopausal syndrome. However, its therapeutic mechanisms are still under investigation, and its effects on gynecologically healthy women remain unknown. This study was conducted to investigate whether and how SMT influences ovarian follicle development by culturing early follicles in vitro and stimulating the ovulation process. SMT dissolved in DPBS was applied at concentrations of 0.1, 1 and 10&#xa0;µg/mL in in vitro maturation medium. Secondary follicles isolated from ovaries of fourteen-day-old female ICR mice were cultured for 8 days in the in vitro maturation medium supplemented with follicle-stimulating hormone. Ovulation was induced in follicles that reached a diameter of 380–450&#xa0;μm. Ovulated oocytes were retrieved, and their spindles and chromosomes were stained to observe meiotic maturation. SMT had no effect on follicle diameter, hormone levels, or survival rates during ovarian follicle development. However, ovulation and the production of mature oocytes were enhanced when SMT was applied during in vitro culture. Additionally, the expression of genes related to progesterone production (<i>lhcgr</i> and <i>hsd3b</i>) was elevated, while the <i>ptgs2</i> gene, which regulates inflammation, was decreased in the SMT-treated group. This study demonstrated that SMT may influence ovarian follicle development by regulating progesterone production and the inflammatory response. Consequently, SMT administration may have the potential to enhance reproductive function, even in non-diseased, fertile women.</p>

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Samul-tang promotes in vitro follicle development by regulating inflammation in healthy, non-diseased young mice

  • 期刊论文

  • 发表日期：

    2025年3月12日

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  • Inyoung Kang,
  • Myoungjoo Koo,
  • Wonmo Lee,
  • Sooseong You,
  • Jin Hyun Jun,
  • Jaewang Lee

摘要

Samul-tang (SMT) is a well-known traditional herbal medicine. Recent studies have reported that SMT exhibits protective biological effects against inflammation, oxidative stress, and cancer. It is commonly used to treat gynecological disorders, including irregular menstruation, pre-eclampsia, and postmenopausal syndrome. However, its therapeutic mechanisms are still under investigation, and its effects on gynecologically healthy women remain unknown. This study was conducted to investigate whether and how SMT influences ovarian follicle development by culturing early follicles in vitro and stimulating the ovulation process. SMT dissolved in DPBS was applied at concentrations of 0.1, 1 and 10 µg/mL in in vitro maturation medium. Secondary follicles isolated from ovaries of fourteen-day-old female ICR mice were cultured for 8 days in the in vitro maturation medium supplemented with follicle-stimulating hormone. Ovulation was induced in follicles that reached a diameter of 380–450 μm. Ovulated oocytes were retrieved, and their spindles and chromosomes were stained to observe meiotic maturation. SMT had no effect on follicle diameter, hormone levels, or survival rates during ovarian follicle development. However, ovulation and the production of mature oocytes were enhanced when SMT was applied during in vitro culture. Additionally, the expression of genes related to progesterone production (lhcgr and hsd3b) was elevated, while the ptgs2 gene, which regulates inflammation, was decreased in the SMT-treated group. This study demonstrated that SMT may influence ovarian follicle development by regulating progesterone production and the inflammatory response. Consequently, SMT administration may have the potential to enhance reproductive function, even in non-diseased, fertile women.