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<p>Brown rot is one of the major postharvest diseases of Chinese cherry, causing fruit browning and decay and severely reducing its economic value. Hydrogen-rich water (HRW) can prolong fruit shelf life, improve fruit quality, and enhance disease resistance, but its mechanism in conferring resistance to brown rot in Chinese cherry remains unclear. In this study, fruit quality analysis, transcriptomics, and metabolomics were used to investigate the response mechanism of HRW against brown rot in Chinese cherry. The results showed that HRW treatment delayed lesion expansion, reduced the disease index and weight loss rate, and the 25% HRW treatment exhibited the best effect. HRW treatment enhanced the activities of POD, PPO, PAL, CHI, and GLU in cherry fruit. Omics analyses identified 340 differentially expressed genes and 223 differential metabolites. The differentially expressed genes were mainly enriched in pathways such as photosynthesis–antenna proteins, phenylpropanoid biosynthesis, flavonoid biosynthesis, and plant hormone signal transduction. The differential metabolites were mainly enriched in flavone and flavonol biosynthesis, purine metabolism, alanine, aspartate and glutamate metabolism, and linoleic acid metabolism. Integrated omics analysis indicated that the HRW-induced resistance mechanism against brown rot in Chinese cherry was primarily achieved through the upregulation of key genes in the phenylpropanoid and flavonoid biosynthesis pathways, including <i>4CL</i>,<i> CCR</i>,<i> BGLU</i>,<i> C3’H</i>,<i> CAD</i>,<i> CHS</i>,<i> CHI</i>, and <i>DFR</i>, as well as the increased accumulation of coniferin and 3-acetyl-3,4,5-trihydroxyflavone. Therefore, HRW enhances resistance to brown rot in Chinese cherry mainly by regulating the phenylpropanoid and flavonoid biosynthesis pathways.</p>

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Integrated transcriptomic and metabolomic analysis of the effects of hydrogen-rich water treatment on brown rot resistance in Chinese cherry fruit

  • 期刊论文

  • 发表日期：

    2026年7月10日

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  • Qing Luo,
  • Yongfei Wu,
  • Manjing Lian,
  • Kaizhen Luo,
  • Fengmei He,
  • Chunya Jiang,
  • Xuelian Yang

摘要

Brown rot is one of the major postharvest diseases of Chinese cherry, causing fruit browning and decay and severely reducing its economic value. Hydrogen-rich water (HRW) can prolong fruit shelf life, improve fruit quality, and enhance disease resistance, but its mechanism in conferring resistance to brown rot in Chinese cherry remains unclear. In this study, fruit quality analysis, transcriptomics, and metabolomics were used to investigate the response mechanism of HRW against brown rot in Chinese cherry. The results showed that HRW treatment delayed lesion expansion, reduced the disease index and weight loss rate, and the 25% HRW treatment exhibited the best effect. HRW treatment enhanced the activities of POD, PPO, PAL, CHI, and GLU in cherry fruit. Omics analyses identified 340 differentially expressed genes and 223 differential metabolites. The differentially expressed genes were mainly enriched in pathways such as photosynthesis–antenna proteins, phenylpropanoid biosynthesis, flavonoid biosynthesis, and plant hormone signal transduction. The differential metabolites were mainly enriched in flavone and flavonol biosynthesis, purine metabolism, alanine, aspartate and glutamate metabolism, and linoleic acid metabolism. Integrated omics analysis indicated that the HRW-induced resistance mechanism against brown rot in Chinese cherry was primarily achieved through the upregulation of key genes in the phenylpropanoid and flavonoid biosynthesis pathways, including 4CL, CCR, BGLU, C3’H, CAD, CHS, CHI, and DFR, as well as the increased accumulation of coniferin and 3-acetyl-3,4,5-trihydroxyflavone. Therefore, HRW enhances resistance to brown rot in Chinese cherry mainly by regulating the phenylpropanoid and flavonoid biosynthesis pathways.