<p>One of the key environmental elements that influences plant growth in vitro is light quality. Currently, a variety of horticultural plants are regenerated in vitro using light-emitting diode (LED) light sources to produce healthy, high-quality plants that can adapt well to ex vitro transplantation conditions. Investigating the impact of various spectrum light sources at various phases of in vitro regeneration is essential, though. The objective of this research was to examine how red (R), blue (B), white (W), red plus blue (RB, 1:1), red, green (G), and blue (RGB, 1:1:1) LEDs affect the growth of protocorm-like bodies (PLBs), shoot regeneration, and the rooting stages of shoots. The findings showed that B-LEDs were accountable for PLB proliferation, whereas R-LEDs were responsible for increased shoot regeneration and improved growth matrices with shoots and plantlets as compared to other LED treatments. Plant height, leaf count, and dry matter percentage were all higher in the plantlets that were regenerated under R-LED. On the other hand, more root regeneration and longer roots were caused by the B-LED treatment. Plants cultivated under RB LEDs had greater levels of carotenoid pigments, total chlorophyll, chlorophyll a, and chlorophyll b. When compared to other treatments, photosynthetic fluorescence characteristics like maximum quantum yield of PSII (Fv/Fm), photochemical quenching coefficient (qP), relative electron transport in PSII (ETRII), and non-photochemical quenching (NPQ) were lower in plants cultivated under R-LED treatment. The best LED for in vitro <i>Cymbidium</i> ‘Snow Pearl’ plant regeneration was as follows: The B-LED was good for PLB proliferation, the R-LED was appropriate during shoot regeneration, and the growth of plantlets, the physiological characteristics such as chlorophyll a, chlorophyll b, total chlorophyll, and carotenoid content and number of epidermal cells per unit area were optimum with the plants grown under RB LED light.</p>