Scholarlink

<p>This study investigated the metabolic basis of color differentiation in <i>Curcuma alismatifolia</i> sterile bracts using liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based widely targeted metabolomics. We identified 1034 metabolites across different colored bract regions, with predominant classes including organic acids, amino acids and derivatives, flavonoids, lipids, terpenoids, organic heterocyclic compounds, and phenolic acids. Statistical analysis (VIP &gt; 1, <i>p</i> &lt; 0.05) revealed 118 and 104 differentially accumulated metabolites in two variety comparisons, with 61 common metabolites. Pathway analysis identified amino acid metabolism and organic acid metabolism as the most significantly altered pathways. Further analysis of the variegated regions demonstrated significant accumulation of succinic anhydride, <i>L</i>-tryptophan, <i>DL</i>-indole-3-lactic acid, uridine 5’-diphosphate galactose, and uridine 5’-diphosphate glucose, along with specific enrichment of various anthocyanin derivatives including malvidin-3-galactoside chloride, oenin chloride, and keracyanin chloride. Our results indicate that L-tryptophan, citric acid, and isocitrate are consistently associated with bract differentiation, potentially through mechanisms such as precursor supply and vacuolar pH regulation that require further validation. These findings provide insights into the metabolic framework underlying <i>C. alismatifolia</i> bract coloration, offering perspectives on Zingiberaceae pigmentation mechanisms and identifying potential biomarkers for ornamental plant breeding. This work provides information for the molecular characterization of this horticulturally important species and suggests candidate metabolic engineering strategies that warrant further investigation for trait improvement.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Widely targeted metabolomics reveals metabolite differences in sterile bract variegation of Curcuma alismatifolia

  • 期刊论文

  • 发表日期：

    2026年4月15日

查看全文
  • Qi Jiang,
  • Can Tao,
  • Weiwei Huang,
  • Huiwen Yu,
  • Luanmei Lu

摘要

This study investigated the metabolic basis of color differentiation in Curcuma alismatifolia sterile bracts using liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based widely targeted metabolomics. We identified 1034 metabolites across different colored bract regions, with predominant classes including organic acids, amino acids and derivatives, flavonoids, lipids, terpenoids, organic heterocyclic compounds, and phenolic acids. Statistical analysis (VIP > 1, p < 0.05) revealed 118 and 104 differentially accumulated metabolites in two variety comparisons, with 61 common metabolites. Pathway analysis identified amino acid metabolism and organic acid metabolism as the most significantly altered pathways. Further analysis of the variegated regions demonstrated significant accumulation of succinic anhydride, L-tryptophan, DL-indole-3-lactic acid, uridine 5’-diphosphate galactose, and uridine 5’-diphosphate glucose, along with specific enrichment of various anthocyanin derivatives including malvidin-3-galactoside chloride, oenin chloride, and keracyanin chloride. Our results indicate that L-tryptophan, citric acid, and isocitrate are consistently associated with bract differentiation, potentially through mechanisms such as precursor supply and vacuolar pH regulation that require further validation. These findings provide insights into the metabolic framework underlying C. alismatifolia bract coloration, offering perspectives on Zingiberaceae pigmentation mechanisms and identifying potential biomarkers for ornamental plant breeding. This work provides information for the molecular characterization of this horticulturally important species and suggests candidate metabolic engineering strategies that warrant further investigation for trait improvement.