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<p>Glutathione peroxidase (<i>GPX</i>) is a key antioxidant enzyme that maintains hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) homeostasis and participates in abiotic stress and hormone responses in plants. The <i>GPX</i> family has diversified across plant species, contributing to adaptation to various stress conditions. In this study, <i>GPX</i> family genes in <i>Brassica rapa</i> (<i>B. rapa</i>) were identified through genome-wide analysis. For each <i>BrGPX</i> member, we compiled detailed information including chromosomal location, gene and protein lengths, gene structure, molecular weight, and isoelectric point. Phylogenetic and collinearity analyses revealed that homologous genes clustered together among <i>B. rapa</i>, <i>Arabidopsis thaliana</i> (<i>A. thaliana</i>), <i>Brassica napus (B. napus),</i> and <i>Brassica oleracea</i> (<i>B. oleracea</i>). To predict biological functions, cis-acting elements in <i>BrGPX</i> promoters were analyzed, revealing diverse hormone-responsive and abiotic stress-related motifs. Expression profiling showed tissue-specific patterns in roots, leaves, flowers, siliques, and stems, as well as differential responses to abscisic acid (ABA), jasmonic acid (JA), indole-3-acetic acid (IAA), FeSO4, high salinity, drought, and temperature stresses. Furthermore, overexpression of <i>BrGPX1</i> and <i>BrGPX7</i> in yeast and <i>A. thaliana</i> enhanced tolerance to drought and salt stress, indicating that <i>BrGPX</i> members play positive roles in abiotic stress adaptation.</p>

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Genome-Wide analysis of GPX family in brassica rapa and the molecular insight of GPX1 and 7 response to abiotic stresses

  • 期刊论文

  • 发表日期：

    2025年10月27日

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  • Xiujuan Dong,
  • Chuang Liang,
  • Qingchang Feng,
  • Yaowei Zhang,
  • Yan Liu

摘要

Glutathione peroxidase (GPX) is a key antioxidant enzyme that maintains hydrogen peroxide (H2O2) homeostasis and participates in abiotic stress and hormone responses in plants. The GPX family has diversified across plant species, contributing to adaptation to various stress conditions. In this study, GPX family genes in Brassica rapa (B. rapa) were identified through genome-wide analysis. For each BrGPX member, we compiled detailed information including chromosomal location, gene and protein lengths, gene structure, molecular weight, and isoelectric point. Phylogenetic and collinearity analyses revealed that homologous genes clustered together among B. rapa, Arabidopsis thaliana (A. thaliana), Brassica napus (B. napus), and Brassica oleracea (B. oleracea). To predict biological functions, cis-acting elements in BrGPX promoters were analyzed, revealing diverse hormone-responsive and abiotic stress-related motifs. Expression profiling showed tissue-specific patterns in roots, leaves, flowers, siliques, and stems, as well as differential responses to abscisic acid (ABA), jasmonic acid (JA), indole-3-acetic acid (IAA), FeSO4, high salinity, drought, and temperature stresses. Furthermore, overexpression of BrGPX1 and BrGPX7 in yeast and A. thaliana enhanced tolerance to drought and salt stress, indicating that BrGPX members play positive roles in abiotic stress adaptation.