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<p>The dried roots of <i>Ampelopsis delavayana</i> Planchon ex Franchet and <i>Ampelopsis glandulosa</i> (Wallich) Momiyama var. <i>heterophylla</i> (Thunberg) Momiyama are valuable medicinal materials widely used by ethnic minorities in Guizhou. However, the similar morphological characteristics within the <i>Ampelopsis</i> genus make accurate identification challenging. To ensure the safety of clinical medication, it is crucial to establish an effective identification method. The complete chloroplast genomes of <i>A. delavayana</i> and <i>A. glandulosa</i> var. <i>heterophylla</i> were sequenced, assembled, and annotated, and 9 other <i>Ampelopsis</i> chloroplast genome sequences were used for comparative analysis as well as 63 complete chloroplast genome sequences from the subfamily Vitoideae for phylogenetic analysis. The chloroplast genomes of <i>A. delavayana</i> and <i>A. glandulosa</i> var. <i>heterophylla</i> exhibited a typical quadripartite structure with a conserved genome comprising 162,415 and 162,302 bp, respectively. Gene annotations of the two species revealed 115 single-copy genes, with 19 genes duplicated in inverted repeat regions and 18 genes possessing introns. Comparative analysis of the two complete chloroplast genomes with the other nine <i>Ampelopsis</i> sequences revealed higher sequence variation in single-copy regions than in inverted repeat regions. Phylogenetic analysis with high bootstrap values revealed that <i>Ampelopsis</i> species, including <i>A. delavayana</i> and <i>A. glandulosa</i> var. <i>heterophylla</i>, formed a clade and were effectively distinguished within the tribe Ampelopsideae. In this study, <i>A. delavayana</i> and <i>A. glandulosa</i> var. <i>heterophylla</i> were effectively distinguished from related species in the genus <i>Ampelopsis</i>, which may provide insight into the evolution, genetic structure, and development of chloroplast DNA markers of <i>Ampelopsis</i>.</p>

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Characterization and phylogenetic analysis of chloroplast genomes in Ampelopsis delavayana and Ampelopsis glandulosa var. heterophylla (Vitaceae)

  • 期刊论文

  • 发表日期：

    2025年6月10日

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  • Chun-mei Yang,
  • Bo Wang,
  • Jing-zhong Chen,
  • Qing-wen Sun,
  • Ze-huan Wang,
  • Yuan Huang

摘要

The dried roots of Ampelopsis delavayana Planchon ex Franchet and Ampelopsis glandulosa (Wallich) Momiyama var. heterophylla (Thunberg) Momiyama are valuable medicinal materials widely used by ethnic minorities in Guizhou. However, the similar morphological characteristics within the Ampelopsis genus make accurate identification challenging. To ensure the safety of clinical medication, it is crucial to establish an effective identification method. The complete chloroplast genomes of A. delavayana and A. glandulosa var. heterophylla were sequenced, assembled, and annotated, and 9 other Ampelopsis chloroplast genome sequences were used for comparative analysis as well as 63 complete chloroplast genome sequences from the subfamily Vitoideae for phylogenetic analysis. The chloroplast genomes of A. delavayana and A. glandulosa var. heterophylla exhibited a typical quadripartite structure with a conserved genome comprising 162,415 and 162,302 bp, respectively. Gene annotations of the two species revealed 115 single-copy genes, with 19 genes duplicated in inverted repeat regions and 18 genes possessing introns. Comparative analysis of the two complete chloroplast genomes with the other nine Ampelopsis sequences revealed higher sequence variation in single-copy regions than in inverted repeat regions. Phylogenetic analysis with high bootstrap values revealed that Ampelopsis species, including A. delavayana and A. glandulosa var. heterophylla, formed a clade and were effectively distinguished within the tribe Ampelopsideae. In this study, A. delavayana and A. glandulosa var. heterophylla were effectively distinguished from related species in the genus Ampelopsis, which may provide insight into the evolution, genetic structure, and development of chloroplast DNA markers of Ampelopsis.