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<p>Basic helix-loop-helix (<i>bHLH</i>) TFs are key to stress response, growth, and development of cabbage, a vegetable crop with considerable economic and nutritional importance globally. The study classified the cabbage bHLH gene family into 23 groups, each containing 244 unevenly distributed <i>BobHLH</i> genes across nine chromosomes. These genes encode 84 to 1037 amino acids, most of which are hydrophilic. Moreover, it was not limited to the prediction of functions and structures for these genes. Expression analysis under abiotic stresses was conducted to identify the functional and evolutionary relationships in the cabbage genome. Promoter and Gene ontology analysis showed the presence of several abiotic stress-responsive cis-regulatory elements in the <i>BobHLH</i> promoter. Most of the identified <i>BobHLH</i> genes were found to be localized in the nucleus, with the majority exhibiting tissue-specific expression patterns. Different abiotic stresses negatively regulate cabbage plant growth at various parameters, including shoot and root fresh and dry weights. Furthermore, RT-qPCR analysis revealed the stress-induced expression of nine <i>BobHLH</i> genes in response to cold, heat, NaCl, and PEG. The study thus deduces that these genes further underscore the enhancement potential of cabbage resistance to abiotic stresses and offer valuable targets for genetic improvement and breeding strategies.</p>

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Identification and analysis of abiotic stress-responsive bHLH gene family in Brassica oleracea

  • 期刊论文

  • 发表日期：

    2025年4月11日

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  • Muhammad Adnan Raza,
  • Qin Wenjun,
  • Song Jianghua

摘要

Basic helix-loop-helix (bHLH) TFs are key to stress response, growth, and development of cabbage, a vegetable crop with considerable economic and nutritional importance globally. The study classified the cabbage bHLH gene family into 23 groups, each containing 244 unevenly distributed BobHLH genes across nine chromosomes. These genes encode 84 to 1037 amino acids, most of which are hydrophilic. Moreover, it was not limited to the prediction of functions and structures for these genes. Expression analysis under abiotic stresses was conducted to identify the functional and evolutionary relationships in the cabbage genome. Promoter and Gene ontology analysis showed the presence of several abiotic stress-responsive cis-regulatory elements in the BobHLH promoter. Most of the identified BobHLH genes were found to be localized in the nucleus, with the majority exhibiting tissue-specific expression patterns. Different abiotic stresses negatively regulate cabbage plant growth at various parameters, including shoot and root fresh and dry weights. Furthermore, RT-qPCR analysis revealed the stress-induced expression of nine BobHLH genes in response to cold, heat, NaCl, and PEG. The study thus deduces that these genes further underscore the enhancement potential of cabbage resistance to abiotic stresses and offer valuable targets for genetic improvement and breeding strategies.