<p>In breeding, although superior parents are generally selected for cross-hybridization, the pollen of elite cultivars may not always be available. Stored pollen provides a practical solution when freshly harvested pollen is unavailable, ensuring its viability for breeding purposes. In this study, we evaluated pollinia of the orchid hybrid cultivar <i>Phalaenopsis</i> ‘KS Little Gem’ at nine different flowering stages with respect to viability after storage for up to 24 months at different temperatures based on in vitro post-storage pollen germination. We detected considerable variation in pollen germination with respect to flowering stage, storage temperature, and storage duration. Pollen collected at flowering stages 1 to 6 and stored at all assessed temperatures for 2 months remained viable. At all storage temperatures, the highest germination was observed in pollen collected from stage 1 after 1 month of storage. Germination of pollen collected at all flowering stages and stored at all assessed temperatures declined significantly with storage prolongation. Pollen stored at 4^°C for longer than 2 months showed a marked loss of viability. At -20^°C, pollen collected at stages 1 to 3 remained viable until 12 months, whereas at -80^°C, germination of pollen obtained at flowering stages 1 to 5 was observed up until 18 months of storage, although only stage 1 pollen stored at -80^°C remained viable after 24 months. Our findings indicate that <i>Phalaenopsis</i> ‘KS Little Gem’ pollen can be stored at -20^°C for up to 12 months for short-term storage and at -80^°C for up to 24 months for long-term storage.</p>