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<p>Pear trees typically exhibit a prolonged juvenile period, which significantly restricts the breeding process. Additionally, the increasing prevalence of soil salinization further constrains pear production. The nuclear factor Y (NF-Y) represents a crucial family of transcription factors that play vital roles in plant development and responses to abiotic stress. However, research on NF-Y members in Pyrus is currently limited. In this study, utilizing the released genome assembly, we identified a total of 41 PbNF-Y encoding genes in Chinese white pear (<i>Pyrus bretschneideri</i> Rehd), comprising 11 <i>PbNF-YAs</i>, 20 <i>PbNF-YBs</i>, and 10 <i>PbNF-YCs</i>, which were named according to the standardized nomenclature in <i>Arabidopsis</i>. Phylogenetic analysis, gene structure evaluation, and sequence alignments indicated the conservation of <i>PbNF-Ys</i> throughout the evolutionary process in Pyrus. Based on previous reports and transcriptome database analyses related to the juvenile-adult phase transition, <i>NF-YA4a</i> was identified as a candidate gene for further investigation. Quantitative PCR (qPCR) results revealed that the expression of <i>NF-YA4a</i> increased during ontogeny, reaching a peak at the adult phase. Furthermore, <i>NF-YA4a</i> exhibited a rapid response to NaCl and ABA treatments, and was also induced by dehydration, but showed no response to low temperature. Overexpression of <i>NF-YA4a</i> in <i>Arabidopsis</i> led to earlier production of abaxial trichomes and flowering phenotypes, as well as enhanced salt stress tolerance compared to the wild-type phenotype. These findings suggest that <i>NF-YA4a</i> positively regulates the juvenile-adult phase transition and salt stress tolerance in Pyrus. This research may provide a foundational basis for future studies on the regulatory mechanisms involved in juvenile-adult phase changes and salt stress responses in Pyrus trees.</p>

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Nuclear factor NF-YA4a regulates salt stress tolerance and juvenile-adult phase transition in Pyrus

  • 期刊论文

  • 发表日期：

    2025年1月17日

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  • Yingjie Yang,
  • Yuling Mei,
  • Xueping Li,
  • Ran Wang,
  • Chunhui Ma,
  • Jiankun Song,
  • Zhenhua Cui,
  • Jianlong Liu,
  • Xiaozhi Zhou,
  • Dingli Li

摘要

Pear trees typically exhibit a prolonged juvenile period, which significantly restricts the breeding process. Additionally, the increasing prevalence of soil salinization further constrains pear production. The nuclear factor Y (NF-Y) represents a crucial family of transcription factors that play vital roles in plant development and responses to abiotic stress. However, research on NF-Y members in Pyrus is currently limited. In this study, utilizing the released genome assembly, we identified a total of 41 PbNF-Y encoding genes in Chinese white pear (Pyrus bretschneideri Rehd), comprising 11 PbNF-YAs, 20 PbNF-YBs, and 10 PbNF-YCs, which were named according to the standardized nomenclature in Arabidopsis. Phylogenetic analysis, gene structure evaluation, and sequence alignments indicated the conservation of PbNF-Ys throughout the evolutionary process in Pyrus. Based on previous reports and transcriptome database analyses related to the juvenile-adult phase transition, NF-YA4a was identified as a candidate gene for further investigation. Quantitative PCR (qPCR) results revealed that the expression of NF-YA4a increased during ontogeny, reaching a peak at the adult phase. Furthermore, NF-YA4a exhibited a rapid response to NaCl and ABA treatments, and was also induced by dehydration, but showed no response to low temperature. Overexpression of NF-YA4a in Arabidopsis led to earlier production of abaxial trichomes and flowering phenotypes, as well as enhanced salt stress tolerance compared to the wild-type phenotype. These findings suggest that NF-YA4a positively regulates the juvenile-adult phase transition and salt stress tolerance in Pyrus. This research may provide a foundational basis for future studies on the regulatory mechanisms involved in juvenile-adult phase changes and salt stress responses in Pyrus trees.