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<p>Currently, the ex vivo expansion of hematopoietic stem/progenitor cells (HS/PCs) is one of the effective ways to address the shortage of HS/PCs for clinical transplantation. Therefore, the optimal in vitro expansion strategy for HS/PCs is a critical issue to be addressed in current researches. In this study, we separated UCB Mononuclear cells (MNCs) and CD34<sup>+</sup> cells, and cultured them for 14&#xa0;days under serum-free conditions (only growth factors added). Then, the effects of the growth factors on HS/PCs expansion were analyzed and compared. The results showed that under short-term culture (&lt; 7&#xa0;days) the number of nucleated cells expanded significantly more in CD34<sup>+</sup> cells than in MNCs, and the content of CD34<sup>+</sup> cells was also significantly greater than that of CD45<sup>+</sup>CD34<sup>+</sup> and CD34<sup>+</sup>CD38<sup>−</sup> cell subpopulations in MNCs. It is confirmed that static suspension culture without serum was unable to promote the expansion of HS/PCs for a long term (14&#xa0;days) and maintain their primitive stem cell activities. Under identical culture conditions, cell products harvested by expansion with CD34<sup>+</sup> cells as the starting cell source outperformed MNCs in terms of hematopoietic stem/progenitor cell yields and hematopoietic reconstitution capacity.</p>

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Expansion characteristics of CD34+ cells derived from umbilical cord blood HS/PCs in single-parameter culture under serum-free static suspension condition

  • 期刊论文

  • 发表日期：

    2025年12月11日

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  • Hezhi Chen,
  • Liying Li,
  • Weiting Guo,
  • Jingjing Zhu,
  • Langyu He,
  • Yuen Yee Cheng,
  • Jie Xu,
  • Yue Kang,
  • Xiujuan Zhang,
  • Kedong Song

摘要

Currently, the ex vivo expansion of hematopoietic stem/progenitor cells (HS/PCs) is one of the effective ways to address the shortage of HS/PCs for clinical transplantation. Therefore, the optimal in vitro expansion strategy for HS/PCs is a critical issue to be addressed in current researches. In this study, we separated UCB Mononuclear cells (MNCs) and CD34+ cells, and cultured them for 14 days under serum-free conditions (only growth factors added). Then, the effects of the growth factors on HS/PCs expansion were analyzed and compared. The results showed that under short-term culture (< 7 days) the number of nucleated cells expanded significantly more in CD34+ cells than in MNCs, and the content of CD34+ cells was also significantly greater than that of CD45+CD34+ and CD34+CD38− cell subpopulations in MNCs. It is confirmed that static suspension culture without serum was unable to promote the expansion of HS/PCs for a long term (14 days) and maintain their primitive stem cell activities. Under identical culture conditions, cell products harvested by expansion with CD34+ cells as the starting cell source outperformed MNCs in terms of hematopoietic stem/progenitor cell yields and hematopoietic reconstitution capacity.