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<p>Dopamine (DA) plays a central role in the management of Parkinson’s disease (PD), and the loss or depletion of dopaminergic neurons in the substantia nigra pars compacta (SNpc) is associated with the onset and progression of PD. 3,4-Dihydroxy Phenyl-Alanine (L-DOPA or LD), the precursor of DA, remains the gold-standard therapeutic drug for PD. With the growing interest in plant-derived neuroactive compounds, there is a pressing need for a precise analytical method capable of simultaneously quantifying these molecules. Here, a rapid, precise, and robust reversed-phase Ultra Performance Liquid Chromatography (RP-UPLC) method was developed for the simultaneous estimation of DA and LD. The method was validated according to the International Council for Harmonization (ICH) Q2(R1) guidelines. Validation results demonstrated excellent linearity (R²=1), accuracy ranging from 99 to 101%, and intra- and inter-day precision with Relative Standard Deviation (RSD%) values below 2. LoD values for DA and LD were 0.01 and 0.04&#xa0;µg/mL, respectively, while LoQ values were 0.03 and 0.12&#xa0;µg/mL, respectively. To evaluate the applicability of the method for pharmaceutical quality assessment and high-throughput screening of plant materials, a commercially available LD tablet and tissues from ten Faba bean genotypes were analyzed. The genotype- and tissue-specific accumulation patterns of DA and LD revealed that leaf and seed tissues, respectively, can serve as promising natural sources for bio-sourcing. These variations underscore the importance of screening diverse Faba bean germplasm to identify suitable genotypes for developing plant-based functional foods and formulations aimed at supporting PD management.</p>

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Development and validation of a rapid RP-UPLC method for simultaneous quantification of L-DOPA and dopamine in Faba bean (Vicia Faba L.) tissues

  • 期刊论文

  • 发表日期：

    2026年2月24日

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  • Mohammad Danish Jawed Ansari,
  • Rakesh Kumar Sinha,
  • Sujit Kumar Bishi

摘要

Dopamine (DA) plays a central role in the management of Parkinson’s disease (PD), and the loss or depletion of dopaminergic neurons in the substantia nigra pars compacta (SNpc) is associated with the onset and progression of PD. 3,4-Dihydroxy Phenyl-Alanine (L-DOPA or LD), the precursor of DA, remains the gold-standard therapeutic drug for PD. With the growing interest in plant-derived neuroactive compounds, there is a pressing need for a precise analytical method capable of simultaneously quantifying these molecules. Here, a rapid, precise, and robust reversed-phase Ultra Performance Liquid Chromatography (RP-UPLC) method was developed for the simultaneous estimation of DA and LD. The method was validated according to the International Council for Harmonization (ICH) Q2(R1) guidelines. Validation results demonstrated excellent linearity (R²=1), accuracy ranging from 99 to 101%, and intra- and inter-day precision with Relative Standard Deviation (RSD%) values below 2. LoD values for DA and LD were 0.01 and 0.04 µg/mL, respectively, while LoQ values were 0.03 and 0.12 µg/mL, respectively. To evaluate the applicability of the method for pharmaceutical quality assessment and high-throughput screening of plant materials, a commercially available LD tablet and tissues from ten Faba bean genotypes were analyzed. The genotype- and tissue-specific accumulation patterns of DA and LD revealed that leaf and seed tissues, respectively, can serve as promising natural sources for bio-sourcing. These variations underscore the importance of screening diverse Faba bean germplasm to identify suitable genotypes for developing plant-based functional foods and formulations aimed at supporting PD management.