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<p>The PYR/PYL/RCAR (PYL) proteins, which function as direct receptors of abscisic acid (ABA), play pivotal regulatory roles in ABA signaling transduction. To investigate the phylogenetic relationships and expression patterns of <i>PYL</i> genes in Eucalyptus, we conducted a genome-wide identification and bioinformatics analysis, followed by qRT-PCR to quantify their expression levels under salt stress and treatments with salicylic and jasmonic acid at 0, 1, 6, 24, and 168&#xa0;h. Based on phylogenetic and structural characteristics, we identified a total of 32 Eucalyptus <i>PYL</i> genes, which are classified into 3 subfamilies (A, B, C) with 3, 8, and 21 members, respectively. Physicochemical property analysis revealed that all Eucalyptus PYL proteins are hydrophilic, with amino acid lengths ranging from 72 to 272 aa, molecular weights between 8.22 and 29.94&#xa0;kDa, and isoelectric points spanning 4.44 to 9.10. With cis-acting element analysis, we identified stress-responsive motifs such as MYB, NAC, WRKY, TCP, and ZF-HD in the promoter regions of the <i>PYL</i> genes. Using expression profiling, we further demonstrate that among 6 selected <i>PYL</i> genes, only <i>EgPYL26</i> exhibits a consistent upregulation trend across all treatments. Other genes, such as <i>EgPYL</i>24, were induced by the hormones, reaching peak expression levels at 24&#xa0;h post-treatment with significant increases compared to the 0-hour baseline. The subcellular localization experiment indicates that <i>EgPYL26</i> is located in the nucleus. Our findings highlight <i>EgPYL26</i> as a key candidate gene for Eucalyptus responses to hormonal and salt stress. Our study establishes a foundation for further functional research and breeding applications of drought-resistant <i>PYL</i> genes in Eucalyptus.</p>

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Genome-wide identification and expression analysis of the PYL gene family in Eucalyptus grandis

  • 期刊论文

  • 发表日期：

    2026年3月5日

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  • Xiaodong Yan,
  • Chunhui Liang,
  • Guiping Mou,
  • Ranling Zuo,
  • Min Huang,
  • Bing Zhang

摘要

The PYR/PYL/RCAR (PYL) proteins, which function as direct receptors of abscisic acid (ABA), play pivotal regulatory roles in ABA signaling transduction. To investigate the phylogenetic relationships and expression patterns of PYL genes in Eucalyptus, we conducted a genome-wide identification and bioinformatics analysis, followed by qRT-PCR to quantify their expression levels under salt stress and treatments with salicylic and jasmonic acid at 0, 1, 6, 24, and 168 h. Based on phylogenetic and structural characteristics, we identified a total of 32 Eucalyptus PYL genes, which are classified into 3 subfamilies (A, B, C) with 3, 8, and 21 members, respectively. Physicochemical property analysis revealed that all Eucalyptus PYL proteins are hydrophilic, with amino acid lengths ranging from 72 to 272 aa, molecular weights between 8.22 and 29.94 kDa, and isoelectric points spanning 4.44 to 9.10. With cis-acting element analysis, we identified stress-responsive motifs such as MYB, NAC, WRKY, TCP, and ZF-HD in the promoter regions of the PYL genes. Using expression profiling, we further demonstrate that among 6 selected PYL genes, only EgPYL26 exhibits a consistent upregulation trend across all treatments. Other genes, such as EgPYL24, were induced by the hormones, reaching peak expression levels at 24 h post-treatment with significant increases compared to the 0-hour baseline. The subcellular localization experiment indicates that EgPYL26 is located in the nucleus. Our findings highlight EgPYL26 as a key candidate gene for Eucalyptus responses to hormonal and salt stress. Our study establishes a foundation for further functional research and breeding applications of drought-resistant PYL genes in Eucalyptus.