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<p>This study aimed to establish an efficient regeneration protocol for high multiplication rates in pineapple (<i>Ananas comosus</i> L., Hybrid ‘MD2’) through somatic organogenesis. We developed a somatic embryogenesis protocol and investigated the effects of different culture medium components on the induction and development of somatic embryos. Nodular callus was initiated from 4-week-old slip explants on a callus induction medium, achieving an 88.12% rate of embryogenic callus induction using 3.0 mg/L BAP. Organized callus formation and somatic embryo development were achieved by transferring callus clumps to embryo induction medium with the same concentration of BAP. Within four weeks, up to 98% of somatic embryos developed into complete plants on basal MS medium with 1.0 mg/L BAP and 500 mg/L glutamine. The acclimatized plantlets showed a 98% survival rate in the greenhouse and produced an average of 85 ± 0.6 well-developed offspring, which were successfully established in the open field and yielded normal fruits. SSR analysis revealed identical banding patterns at four loci, indicating no detectable somaclonal variation. Previous pineapple somatic embryogenesis systems have shown limited regeneration efficiency, multiplication rates, and genetic fidelity assessment, necessitating an improved protocol that ensures rapid, high-quality, and true-to-type plant production. The present efficient protocol provides a valuable platform for high-quality pineapple germplasm, enabling large-scale propagation and genetic manipulation. It may also support research on stress biology and contribute to breeding climate-resilient pineapple cultivars.</p>

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An efficient micropropagation protocol for Ananas comosus L. (Merr. Hybrid “MD2”) through somatic embryogenesis

  • 期刊论文

  • 发表日期：

    2026年3月6日

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  • Tushar Ranjan,
  • Awadhesh Kumar Pal,
  • Hidayatullah Mir,
  • Md. Shamim,
  • Ravi Ranjan Kumar,
  • Mahesh Kumar,
  • Bishun Deo Prasad,
  • Kumari Rajani,
  • Sanskriti Shiwani,
  • Niharika,
  • Parveen Fatima

摘要

This study aimed to establish an efficient regeneration protocol for high multiplication rates in pineapple (Ananas comosus L., Hybrid ‘MD2’) through somatic organogenesis. We developed a somatic embryogenesis protocol and investigated the effects of different culture medium components on the induction and development of somatic embryos. Nodular callus was initiated from 4-week-old slip explants on a callus induction medium, achieving an 88.12% rate of embryogenic callus induction using 3.0 mg/L BAP. Organized callus formation and somatic embryo development were achieved by transferring callus clumps to embryo induction medium with the same concentration of BAP. Within four weeks, up to 98% of somatic embryos developed into complete plants on basal MS medium with 1.0 mg/L BAP and 500 mg/L glutamine. The acclimatized plantlets showed a 98% survival rate in the greenhouse and produced an average of 85 ± 0.6 well-developed offspring, which were successfully established in the open field and yielded normal fruits. SSR analysis revealed identical banding patterns at four loci, indicating no detectable somaclonal variation. Previous pineapple somatic embryogenesis systems have shown limited regeneration efficiency, multiplication rates, and genetic fidelity assessment, necessitating an improved protocol that ensures rapid, high-quality, and true-to-type plant production. The present efficient protocol provides a valuable platform for high-quality pineapple germplasm, enabling large-scale propagation and genetic manipulation. It may also support research on stress biology and contribute to breeding climate-resilient pineapple cultivars.