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<p>An essential component of a plant’s natural defense against infections is its pre-existing phytochemicals. In this work, we used Gas Chromatography-Mass Spectrometry to profile the bioactive metabolite composition of the cucumber line PI-197087, which shows some level of resistance to downy mildew. From healthy leaf extracts, forty metabolites were found. Nineteen of these compounds were employed in an in-silico molecular docking research against two important effector proteins (COXII and QNE V4) from the pathogen <i>Pseudoperonospora cubensis</i> in order to examine their protective potential. The findings showed that the proteins’ active sites generated stable connections with substantial binding affinities, especially from anthracene and 1&#xa0;H-Indene derivatives. Additional analysis revealed that these interactions were energetically favorable and involved key hydrogen bonding and hydrophobic contacts, suggesting a strong inhibitory mechanism. These results imply that certain pre-existing phytochemicals in PI-197087 may function as significant inhibitors of pathogen effector proteins, offering a solid biochemical explanation for the resistance that has been reported. This study provides a promising foundation for future breeding strategies aimed at enhancing disease resistance through metabolite profiling and targeted molecular interventions. </p>

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Phytochemicals as intrinsic defenses: profiling and molecular Docking in the downy mildew-resistant cucumber line PI-197087

  • 期刊论文

  • 发表日期：

    2026年1月27日

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  • Kanchan Sharma,
  • Pragya Ranjan,
  • Suruchi Jindal

摘要

An essential component of a plant’s natural defense against infections is its pre-existing phytochemicals. In this work, we used Gas Chromatography-Mass Spectrometry to profile the bioactive metabolite composition of the cucumber line PI-197087, which shows some level of resistance to downy mildew. From healthy leaf extracts, forty metabolites were found. Nineteen of these compounds were employed in an in-silico molecular docking research against two important effector proteins (COXII and QNE V4) from the pathogen Pseudoperonospora cubensis in order to examine their protective potential. The findings showed that the proteins’ active sites generated stable connections with substantial binding affinities, especially from anthracene and 1 H-Indene derivatives. Additional analysis revealed that these interactions were energetically favorable and involved key hydrogen bonding and hydrophobic contacts, suggesting a strong inhibitory mechanism. These results imply that certain pre-existing phytochemicals in PI-197087 may function as significant inhibitors of pathogen effector proteins, offering a solid biochemical explanation for the resistance that has been reported. This study provides a promising foundation for future breeding strategies aimed at enhancing disease resistance through metabolite profiling and targeted molecular interventions.