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<p><i>Santalum album</i> L. (Indian Sandalwood) a valuable tree species known for its essential oil, is facing increasing threats due to climate change, resulting in habitat loss, decline in genetic diversity and rising incidents of spike disease. Further, excessive exploitation has resulted in loss of adaptive gene pool. The present study investigates the molecular mechanisms governing climate adaptation in <i>S. album</i> through integrated analysis of leaf transcriptome and small RNA sequence datasets. <i>De novo</i> transcriptome assembly identified 38,516 transcripts which were functionally annotated. Small RNA sequencing identified 61 conserved miRNAs, with Sa-miR156 and Sa-miR166 being the most abundant miRNA in sandalwood leaf. Integrated miRNA-mRNA analysis revealed 6814 miRNA-targeted transcripts, predominantly impacting genes involved in transcription regulation, such as SBP-box transcription factors, Zinc finger proteins, and Leucine-rich repeat domains. qRT-PCR validation of 10 miRNAs and their corresponding 15 target transcripts across four genotypes showed significant regional variation in gene expression. Location with higher rainfall, exhibited increased expression of stress-related genes, while drought-prone region, demonstrated specific miRNA-mediated stress responses. The study confirmed the potential role of miRNAs in adaptation under varying climatic conditions in sandalwood. Identification of post-transcriptionally regulated stress response genes and their associated pathways has elucidated the key mechanisms underlying resilience to climatic variability in sandalwood.</p>

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Post transcriptional regulation of climate adaptive variability in Santalum album L. (Indian Sandalwood)

  • 期刊论文

  • 发表日期：

    2025年9月4日

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  • Muthulakshmi Eswaran,
  • K. Chandramouli Madhuvanthi,
  • Thangaraj Karthick,
  • Aiyar Balasubramanian,
  • Suma Arun Dev,
  • Swathi Balakrishnan,
  • Kandasamy Ulaganathan,
  • Sneha Podicheti,
  • Modhumita Ghosh Dasgupta

摘要

Santalum album L. (Indian Sandalwood) a valuable tree species known for its essential oil, is facing increasing threats due to climate change, resulting in habitat loss, decline in genetic diversity and rising incidents of spike disease. Further, excessive exploitation has resulted in loss of adaptive gene pool. The present study investigates the molecular mechanisms governing climate adaptation in S. album through integrated analysis of leaf transcriptome and small RNA sequence datasets. De novo transcriptome assembly identified 38,516 transcripts which were functionally annotated. Small RNA sequencing identified 61 conserved miRNAs, with Sa-miR156 and Sa-miR166 being the most abundant miRNA in sandalwood leaf. Integrated miRNA-mRNA analysis revealed 6814 miRNA-targeted transcripts, predominantly impacting genes involved in transcription regulation, such as SBP-box transcription factors, Zinc finger proteins, and Leucine-rich repeat domains. qRT-PCR validation of 10 miRNAs and their corresponding 15 target transcripts across four genotypes showed significant regional variation in gene expression. Location with higher rainfall, exhibited increased expression of stress-related genes, while drought-prone region, demonstrated specific miRNA-mediated stress responses. The study confirmed the potential role of miRNAs in adaptation under varying climatic conditions in sandalwood. Identification of post-transcriptionally regulated stress response genes and their associated pathways has elucidated the key mechanisms underlying resilience to climatic variability in sandalwood.