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<p>Bolting is a critical genetic trait observed in cruciferous plants, notably cabbage (<i>Brassica oleracea</i> var. <i>capitata</i>), which is highly susceptible to premature bolting. This phenomenon, influenced by environmental conditions and variety-specific characteristics, negatively impacts yield and quality, thus reducing economic returns. This study aimed to elucidate the molecular mechanisms underlying bolting in cabbage by integrating metabolomic and transcriptomic analyses of two contrasting cultivars: bolting-tolerant Gl19004 (A) and bolting-sensitive Gl19039 (B), examined at both vegetative and reproductive stages. The results revealed distinct metabolite accumulation and transcriptional changes before and after bolting. Correlation analysis identified <i>LHY</i>, <i>CCA1</i>, <i>VIN3</i>, and raffinose as key contributors to vernalization-induced bolting. Six plant hormone signal transduction pathways were involved: abscisic acid, anxin, gibberellin, jasmonic acid, ethylene, and salicylic acid, with key genes such as <i>PYR/PYL</i>, <i>PP2C</i>, <i>SnRK2</i>, <i>AUX</i>, <i>GH3</i>, <i>SAUR</i>, <i>DELLA</i>, <i>TGA</i>, <i>ETR</i>, and <i>EBF</i> playing pivotal roles. Transcription factor analysis revealed that DEGs between developmental stages belonged to bHLH, AP2/ERF, HD-ZIP, bZIP, C2H2, and MYB families, highlighting their involvement in the regulation of cabbage bolting. This study lays a foundation for further elucidation of bolting-related genes and their underlying molecular mechanisms in <i>Brassica oleracea</i> var. <i>capitata</i>.</p>

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Transcriptome and metabolome analyses revealed mechanisms underlying vernalization-induced bolting in two contrasting genotypes of cabbage (Brassica oleracea var. capitata)

  • 期刊论文

  • 发表日期：

    2025年7月25日

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  • Huiju Yang,
  • Jingfeng Hu,
  • Mingwei Chen,
  • Mei Lan,
  • Jiangming He

摘要

Bolting is a critical genetic trait observed in cruciferous plants, notably cabbage (Brassica oleracea var. capitata), which is highly susceptible to premature bolting. This phenomenon, influenced by environmental conditions and variety-specific characteristics, negatively impacts yield and quality, thus reducing economic returns. This study aimed to elucidate the molecular mechanisms underlying bolting in cabbage by integrating metabolomic and transcriptomic analyses of two contrasting cultivars: bolting-tolerant Gl19004 (A) and bolting-sensitive Gl19039 (B), examined at both vegetative and reproductive stages. The results revealed distinct metabolite accumulation and transcriptional changes before and after bolting. Correlation analysis identified LHY, CCA1, VIN3, and raffinose as key contributors to vernalization-induced bolting. Six plant hormone signal transduction pathways were involved: abscisic acid, anxin, gibberellin, jasmonic acid, ethylene, and salicylic acid, with key genes such as PYR/PYL, PP2C, SnRK2, AUX, GH3, SAUR, DELLA, TGA, ETR, and EBF playing pivotal roles. Transcription factor analysis revealed that DEGs between developmental stages belonged to bHLH, AP2/ERF, HD-ZIP, bZIP, C2H2, and MYB families, highlighting their involvement in the regulation of cabbage bolting. This study lays a foundation for further elucidation of bolting-related genes and their underlying molecular mechanisms in Brassica oleracea var. capitata.