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<p>Indian pennywort [<i>Centella asiatica</i> (L.) Urb.] is an herbaceous plant used in traditional medicines as it is enriched with triterpenes. Drought stress negatively affects the productivity (biomass) and quality (centellosides) of this important medicinal plant. Methyl jasmonate (MeJA), a natural plant signal-transducer molecule, is a potential elicitor in regulating secondary metabolites and alleviating drought stress. The aim of this study was to assess the impacts of exogenous foliar MeJA application on morphological, physiological, and biochemical traits as well as total centelloside contents of <i>C. asiatica</i> under water-deficit stress. The experiment consisted of three foliar MeJA application doses [0 (distilled water or control), 125, and 250&#xa0;μM] and two soil water regimes [well-watered (WW; 100% field capacity) and water-deficit (WD; 68.3% field capacity)]. Leaf herbal biomass of <i>C. asiatica</i> was promoted by MeJA application and sustained even when exposed to water-deficit condition. Leaf water content was significantly declined by 8.5% under WD compared with plants grown under WW and without MeJA application; however, it was improved in MeJA-treated plants when subjected to WD, leading to the maintenance of high level of chlorophyll pigments. Leaf gas exchange parameters, notably net photosynthetic rate (P<sub>n</sub>) and transpiration rate (T<sub>r</sub>), were sensitive to WD, leading to their significant drop. Interestingly, P<sub>n</sub> and T<sub>r</sub> in MeJA-treated plants were higher than plants grown without MeJA either with or without WD. Without MeJA application, leaf temperature of plants under WD was increased by 1.73&#xa0;°C over WW, leading to a high level of crop water stress index (169% over WW). Asiaticoside in the leaf tissues was elicited by 125&#xa0;μM (1.9-fold over the control) and 250&#xa0;μM MeJA (2.2-fold over the control) and was also elevated by WD condition. In contrast, madecassoside and asiatic acid were significantly decreased when exposed to WD condition. At 250&#xa0;μM MeJA, total centelloside and centelloside yield were retained even when subjected to WD. Based on the results, the exogenous foliar spray of MeJA at 250&#xa0;μM is recommended for enhancing drought tolerance of <i>C. asiatica</i>.</p>

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Mitigation of water-deficit stress in Indian pennywort (Centella asiatica) using exogenous foliar application of methyl jasmonate

  • 期刊论文

  • 发表日期：

    2025年6月23日

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  • Daonapa Chungloo,
  • Cattarin Theerawitaya,
  • Rujira Tisarum,
  • Patchara Praseartkul,
  • Piyanan Pipatsitee,
  • Sushil Kumar Himanshu,
  • Avishek Datta,
  • Suriyan Cha‑um

摘要

Indian pennywort [Centella asiatica (L.) Urb.] is an herbaceous plant used in traditional medicines as it is enriched with triterpenes. Drought stress negatively affects the productivity (biomass) and quality (centellosides) of this important medicinal plant. Methyl jasmonate (MeJA), a natural plant signal-transducer molecule, is a potential elicitor in regulating secondary metabolites and alleviating drought stress. The aim of this study was to assess the impacts of exogenous foliar MeJA application on morphological, physiological, and biochemical traits as well as total centelloside contents of C. asiatica under water-deficit stress. The experiment consisted of three foliar MeJA application doses [0 (distilled water or control), 125, and 250 μM] and two soil water regimes [well-watered (WW; 100% field capacity) and water-deficit (WD; 68.3% field capacity)]. Leaf herbal biomass of C. asiatica was promoted by MeJA application and sustained even when exposed to water-deficit condition. Leaf water content was significantly declined by 8.5% under WD compared with plants grown under WW and without MeJA application; however, it was improved in MeJA-treated plants when subjected to WD, leading to the maintenance of high level of chlorophyll pigments. Leaf gas exchange parameters, notably net photosynthetic rate (Pn) and transpiration rate (Tr), were sensitive to WD, leading to their significant drop. Interestingly, Pn and Tr in MeJA-treated plants were higher than plants grown without MeJA either with or without WD. Without MeJA application, leaf temperature of plants under WD was increased by 1.73 °C over WW, leading to a high level of crop water stress index (169% over WW). Asiaticoside in the leaf tissues was elicited by 125 μM (1.9-fold over the control) and 250 μM MeJA (2.2-fold over the control) and was also elevated by WD condition. In contrast, madecassoside and asiatic acid were significantly decreased when exposed to WD condition. At 250 μM MeJA, total centelloside and centelloside yield were retained even when subjected to WD. Based on the results, the exogenous foliar spray of MeJA at 250 μM is recommended for enhancing drought tolerance of C. asiatica.