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<p>Metabolites glycosylated by UDP-glycosyltransferases (UGTs) play a crucial role in plant-environment interactions and nutritional dynamics. Therefore, identifying stress-responsive UGTs and their regulatory elements is essential for elucidating plant adaptation mechanisms and engineering stress-resilient crops. This study focuses on the identification and functional characterization of a UGT gene, <i>GgUGT72L11</i>, from <i>Glycyrrhiza glabra</i>, which is implicated in flavonoid glycosylation and cold stress tolerance. Phylogenetic analysis revealed that GgUGT72L11 shares 73.3% sequence identity with a known flavonoid (epicatechin) glucosyltransferase from <i>Medicago truncatula</i> (ACC38470), indicating a role in flavonoid glycosylation. Functional assays, including molecular docking and in-vitro enzymatic studies using flavonoids (kaempferol and quercetin), confirmed the glycosylation activity of GgUGT72L11. Additionally, the enzyme exhibited a broad substrate acceptance, catalyzing glycosylation of various classes of aglycones into their corresponding glycosides. Promoter analysis identified multiple <i>cis</i>-regulatory elements responsive to cold, light, hormones, and other abiotic stresses. Quantitative real-time PCR under 12 different stress treatments demonstrated significant induction of <i>GgUGT72L11</i> under cold stress (4&#xa0;°C), with a 36-fold increase, and a 400 fold upregulation in response to abscisic acid (ABA), underscoring its role in abiotic stress responses. Heterologous transient expression of the <i>GgUGT72L11</i> promoter fused to the GUS reporter gene (<i>pro-UGT72L11::GUS</i>) in <i>Nicotiana benthamiana</i> further confirmed cold-inducible promoter activity. Collectively, these findings demonstrate the dual role of <i>GgUGT72L11</i> in flavonoid glycosylation and cold stress response, indicating an underlying association between the two in plant adaptation. Future studies in understanding bioactive glycosides biosynthesis in response to stress adaptation will be highly useful.</p>

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Identification and functional characterization of a cold-inducible UDP-Glycosyltransferase (GgUGT72L11) from Glycyrrhiza glabra

  • 期刊论文

  • 发表日期：

    2025年6月19日

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  • Shahnawaz Hussain,
  • Bhawna Verma,
  • Malik Muzafar Manzoor,
  • Pooja Goyal,
  • Ritu Devi,
  • Ajai P. Gupta,
  • Manoj K. Dhar,
  • Shakti Kumar Dhiman,
  • Fariha Chowdhary,
  • Debaraj Mukherjee,
  • Suphla Gupta

摘要

Metabolites glycosylated by UDP-glycosyltransferases (UGTs) play a crucial role in plant-environment interactions and nutritional dynamics. Therefore, identifying stress-responsive UGTs and their regulatory elements is essential for elucidating plant adaptation mechanisms and engineering stress-resilient crops. This study focuses on the identification and functional characterization of a UGT gene, GgUGT72L11, from Glycyrrhiza glabra, which is implicated in flavonoid glycosylation and cold stress tolerance. Phylogenetic analysis revealed that GgUGT72L11 shares 73.3% sequence identity with a known flavonoid (epicatechin) glucosyltransferase from Medicago truncatula (ACC38470), indicating a role in flavonoid glycosylation. Functional assays, including molecular docking and in-vitro enzymatic studies using flavonoids (kaempferol and quercetin), confirmed the glycosylation activity of GgUGT72L11. Additionally, the enzyme exhibited a broad substrate acceptance, catalyzing glycosylation of various classes of aglycones into their corresponding glycosides. Promoter analysis identified multiple cis-regulatory elements responsive to cold, light, hormones, and other abiotic stresses. Quantitative real-time PCR under 12 different stress treatments demonstrated significant induction of GgUGT72L11 under cold stress (4 °C), with a 36-fold increase, and a 400 fold upregulation in response to abscisic acid (ABA), underscoring its role in abiotic stress responses. Heterologous transient expression of the GgUGT72L11 promoter fused to the GUS reporter gene (pro-UGT72L11::GUS) in Nicotiana benthamiana further confirmed cold-inducible promoter activity. Collectively, these findings demonstrate the dual role of GgUGT72L11 in flavonoid glycosylation and cold stress response, indicating an underlying association between the two in plant adaptation. Future studies in understanding bioactive glycosides biosynthesis in response to stress adaptation will be highly useful.