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<p>Amongst ~ 70 genes annotated to code for mitochondrial ribosomal protein subunits in <i>Arabidopsis</i>, only seven are located in the mitochondrial genome. These genes are regarded as constitutively expressed and coordinately regulated. The promoters of nuclear encoded mitochondrial ribosomal protein coding genes of plants have not been much investigated. In the present study, we analyzed the promoter of <i>Arabidopsis thaliana Rps14</i> gene through deletion analysis. Six different fragments spanning the intergenic region (IGR) and upstream or downstream sequences were tested for promoter activity using the <i>uidA</i> reporter gene assay. Analysis of GUS expression in transgenic <i>A. thaliana</i> plants carrying different deletion constructs revealed that 347&#xa0;bp N-terminal coding sequences of <i>Rps14</i> are essential for promoter function. Based on GUS expression, <i>Rps14</i> promoter was delineated to a 542&#xa0;bp fragment comprising the IGR, 5′ UTR and 347&#xa0;bp coding region. <i>Rps14</i> promoter drives constitutive gene expression in vegetative and reproductive tissues including seedlings, roots, rosette leaves, cauline leaves, sepals, petals, stamen and ovule. In contrast to widely used CaMV35S promoter, <i>AtRps14</i> promoter is capable of driving gene expression in all tissues including stamen and ovule.</p>

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N-terminal coding sequences of the Arabidopsis thaliana nuclear encoded mitochondrial ribosomal protein subunit gene Rps14 are an integral part of its promoter

  • 期刊论文

  • 发表日期：

    2025年3月27日

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  • Suman Lata,
  • Neetu Singh Kushwah,
  • Aashish Ranjan,
  • Ritesh Kumar Raipuria,
  • Pankaj Kumar,
  • R. Srinivasan,
  • Shripad Ramachandra Bhat

摘要

Amongst ~ 70 genes annotated to code for mitochondrial ribosomal protein subunits in Arabidopsis, only seven are located in the mitochondrial genome. These genes are regarded as constitutively expressed and coordinately regulated. The promoters of nuclear encoded mitochondrial ribosomal protein coding genes of plants have not been much investigated. In the present study, we analyzed the promoter of Arabidopsis thaliana Rps14 gene through deletion analysis. Six different fragments spanning the intergenic region (IGR) and upstream or downstream sequences were tested for promoter activity using the uidA reporter gene assay. Analysis of GUS expression in transgenic A. thaliana plants carrying different deletion constructs revealed that 347 bp N-terminal coding sequences of Rps14 are essential for promoter function. Based on GUS expression, Rps14 promoter was delineated to a 542 bp fragment comprising the IGR, 5′ UTR and 347 bp coding region. Rps14 promoter drives constitutive gene expression in vegetative and reproductive tissues including seedlings, roots, rosette leaves, cauline leaves, sepals, petals, stamen and ovule. In contrast to widely used CaMV35S promoter, AtRps14 promoter is capable of driving gene expression in all tissues including stamen and ovule.