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<p>Drought is a highly stressful environmental factor that limits plant growth and yield. The expressions of drought-responsive genes of sorghum (<i>Sorghum bicolor</i> (L.) Moench) in response to different degrees of drought stress have rarely been investigated. Here, we performed a transcriptome analysis of leaf samples from water-controlled (normal water supply, relative soil water content = 70%, CK; mild drought stress, relative soil water content = 50%, DR1; severe drought stress, relative soil water content = 30%, DR2) sorghum seedlings. The clean reads were obtained and assembled into 34,367 unigenes. Totally 883 and 1582 differentially expressed genes (DEGs) were identified under DR1 and DR2 conditions, respectively. Gene Ontology (GO) annotation showed that DEGs were most significantly enriched into “transcription regulator activity” and “tetrapyrrole binding” under DR1 and DR2 treatments, respectively. According to Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, “carbon fixation in photosynthetic organisms” and “plant hormone signal transduction” were significantly enriched under drought stress. Besides, expression levels of several specific genes related to stress response and carbon metabolism were significantly altered under drought stress. Moreover, 13 and 16 DEGs occurred with differential alternative splicing under DR1 and DR2 conditions, respectively. The findings of this work provide a valuable resource on drought-responsive genes and contribute to the improvement of drought tolerance in sorghum.</p>

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Transcriptome of sorghum (Sorghum bicolor (L.) Moench) in response to drought stress

  • 期刊论文

  • 发表日期：

    2025年2月1日

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  • Junming Ma,
  • Zhuojun Liu,
  • Zihao Guo,
  • Xinyu Wang,
  • Chunlei Zou,
  • Chunlai Zhang

摘要

Drought is a highly stressful environmental factor that limits plant growth and yield. The expressions of drought-responsive genes of sorghum (Sorghum bicolor (L.) Moench) in response to different degrees of drought stress have rarely been investigated. Here, we performed a transcriptome analysis of leaf samples from water-controlled (normal water supply, relative soil water content = 70%, CK; mild drought stress, relative soil water content = 50%, DR1; severe drought stress, relative soil water content = 30%, DR2) sorghum seedlings. The clean reads were obtained and assembled into 34,367 unigenes. Totally 883 and 1582 differentially expressed genes (DEGs) were identified under DR1 and DR2 conditions, respectively. Gene Ontology (GO) annotation showed that DEGs were most significantly enriched into “transcription regulator activity” and “tetrapyrrole binding” under DR1 and DR2 treatments, respectively. According to Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, “carbon fixation in photosynthetic organisms” and “plant hormone signal transduction” were significantly enriched under drought stress. Besides, expression levels of several specific genes related to stress response and carbon metabolism were significantly altered under drought stress. Moreover, 13 and 16 DEGs occurred with differential alternative splicing under DR1 and DR2 conditions, respectively. The findings of this work provide a valuable resource on drought-responsive genes and contribute to the improvement of drought tolerance in sorghum.