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<p>WRKY proteins are plant-specific transcription factors (TFs) that have widespread involvement in plant growth, development, and stress responses. Increasing reports have revealed the essential functions of group III <i>WRKY</i> genes in response to various stresses, whereas no comprehensive information on these genes has been obtained in cucumber. In this work, we identified 6 group III <i>WRKY</i> genes in cucumber using whole-genome analysis. The phylogenetic analysis result indicated that the group III WRKYs in cucumber, cotton, <i>Arabidopsis</i>, and rice can be classified into four clades. Promoter analysis showed that a series of hormone- and stress-related <i>cis</i>-elements were present in the promoter regions of cucumber group III <i>WRKY</i> genes. In addition, RNA-seq analysis revealed that these <i>CsWRKY</i> genes exhibited distinct expression patterns in response to two abiotic stresses (salt and cold) and two biotic stresses (downy mildew and root-knot nematode infection). Further, qRT-PCR results showed that <i>CsWRKY59</i> had differentially changed transcripts under salt, drought, and abscisic acid (ABA) treatments. Yeast assay showed that the full-length CsWRKY59 has transactivation activity. STRING analysis showed that the interaction partners of the CsWRKY59 proteins, including WRKY, MYB, NAC, and other TFs. This is the first report describing cucumber group III <i>WRKY</i> genes and these genes may act as important candidates for the development of stress-resistant varieties in cucumber.</p>

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Identification of the group III WRKY subfamily and preliminary functional characterization of CsWRKY59 in cucumber

  • 期刊论文

  • 发表日期：

    2025年1月31日

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  • Kangli Xia,
  • Zuying Zhou,
  • Yuseng Hu,
  • Yuxuan Wang,
  • Zhaoyang Hu,
  • Yuelong Zhou,
  • Shiqiang Liu,
  • Yong Zhou

摘要

WRKY proteins are plant-specific transcription factors (TFs) that have widespread involvement in plant growth, development, and stress responses. Increasing reports have revealed the essential functions of group III WRKY genes in response to various stresses, whereas no comprehensive information on these genes has been obtained in cucumber. In this work, we identified 6 group III WRKY genes in cucumber using whole-genome analysis. The phylogenetic analysis result indicated that the group III WRKYs in cucumber, cotton, Arabidopsis, and rice can be classified into four clades. Promoter analysis showed that a series of hormone- and stress-related cis-elements were present in the promoter regions of cucumber group III WRKY genes. In addition, RNA-seq analysis revealed that these CsWRKY genes exhibited distinct expression patterns in response to two abiotic stresses (salt and cold) and two biotic stresses (downy mildew and root-knot nematode infection). Further, qRT-PCR results showed that CsWRKY59 had differentially changed transcripts under salt, drought, and abscisic acid (ABA) treatments. Yeast assay showed that the full-length CsWRKY59 has transactivation activity. STRING analysis showed that the interaction partners of the CsWRKY59 proteins, including WRKY, MYB, NAC, and other TFs. This is the first report describing cucumber group III WRKY genes and these genes may act as important candidates for the development of stress-resistant varieties in cucumber.