Development of SCAR marker for authentication in germplasm conservation and SSR & inter-transcribed spacer genetic diversity analysis in medicinally important herb Uraria picta.
摘要
The plant Uraria picta is a well-known medicinal herb with extremely small populations found in Indian geographical regions and has an economically important value for medicine and pharmaceutical purposes but the species has declined sharply mainly due to habitat destruction and its overexploitation for medicinal and aesthetic values from its natural habitat that fetched it in the list of critically endangered species, so its effective conservation strategies are required urgently and moreover there is a limited information about its genuine authentication as the species resembles with similar other related plants and therefore became a little challenging task in classification category. Here we report first SCAR marker (GenBank: MF770709) for its germplasm authentication. Furthermore in the simple sequence repeat (SSR) analysis we find mean Nei’s genetic diversity (1.8704) and lowest Shannon information index (0.5147) among the accessions and in AMOVA analysis we observed variation that shows a little significant value of (P < 0.001). Principle coordinates examination (PCoA) was performed to check the multidimensional relationships between the accessions of U. picta. The PCA graph matches the UPGMA cluster tree that demonstrate close assassinations between the germplasm, however from the inter-transcribed sequence analysis we did not find any major genetic diversity among the accessions. The above mentioned study will be helpful and a way forward in germplasm authentication, conservation and restoration of the above species.