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<p>This work outlines an integrated biorefinery approach for the valorisation of brewer’s spent grain (BSG), targeting protein recovery and poly(3-hydroxybutyrate) (PHB) production. Initially, dextrins and proteins were extracted from BSG, and the residual solids obtained after protein extraction were subsequently subjected to alkaline pretreatment to promote lignin removal and enhance enzymatic hydrolysis. A Central Composite Design was employed to optimise lignin removal while minimising hemicellulose and glucan losses, with optimal conditions determined as 0.71% (w/v) NaOH at 71°C. After 30&#xa0;min of pretreatment under the optimal conditions, lignin, hemicellulose, and glucan removal reached 31.6%, 26.9%, and 12.5%, respectively, while the enzymatic hydrolysis efficiency of glucan and hemicellulose reached 86.5% and 30.0%, respectively. The resulting hydrolysate was efficiently used as a carbon source for PHB production by <i>Paraburkholderia sacchari</i> DSM 17165 under fed-batch conditions, yielding a dry cell weight of 86.6&#xa0;g/L with 49.1% PHB (42.5&#xa0;g/L), corresponding to a PHB yield of&#xa0;0.28&#xa0;g/g and a productivity of&#xa0;1.23&#xa0;g/(L·h). Based on the proposed biorefinery, 1&#xa0;kg of BSG could be used for the production of 76.3&#xa0;g crude protein extract with 45.2% protein purity and 89.7&#xa0;g PHB. The proposed process demonstrates a scalable and resource-efficient strategy for converting brewery residues into biodegradable polymers and protein-rich food extracts, thereby contributing to circular bioeconomy practices.</p>

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Valorisation of brewers’ spent grain via alkaline pretreatment for protein concentrate and poly(3-hydroxybutyrate) production

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年6月25日

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  • Katiana Filippi,
  • Chrysanthi Argeiti,
  • Eleni Anna Kouvelioti,
  • Eleni Stylianou,
  • Olga Psaki,
  • Dimitrios Ladakis,
  • Apostolis Koutinas,
  • Anestis Vlysidis

摘要

This work outlines an integrated biorefinery approach for the valorisation of brewer’s spent grain (BSG), targeting protein recovery and poly(3-hydroxybutyrate) (PHB) production. Initially, dextrins and proteins were extracted from BSG, and the residual solids obtained after protein extraction were subsequently subjected to alkaline pretreatment to promote lignin removal and enhance enzymatic hydrolysis. A Central Composite Design was employed to optimise lignin removal while minimising hemicellulose and glucan losses, with optimal conditions determined as 0.71% (w/v) NaOH at 71°C. After 30 min of pretreatment under the optimal conditions, lignin, hemicellulose, and glucan removal reached 31.6%, 26.9%, and 12.5%, respectively, while the enzymatic hydrolysis efficiency of glucan and hemicellulose reached 86.5% and 30.0%, respectively. The resulting hydrolysate was efficiently used as a carbon source for PHB production by Paraburkholderia sacchari DSM 17165 under fed-batch conditions, yielding a dry cell weight of 86.6 g/L with 49.1% PHB (42.5 g/L), corresponding to a PHB yield of 0.28 g/g and a productivity of 1.23 g/(L·h). Based on the proposed biorefinery, 1 kg of BSG could be used for the production of 76.3 g crude protein extract with 45.2% protein purity and 89.7 g PHB. The proposed process demonstrates a scalable and resource-efficient strategy for converting brewery residues into biodegradable polymers and protein-rich food extracts, thereby contributing to circular bioeconomy practices.