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<p>In this study, the optimization of the solid-liquid extraction of phenolic compounds from grape pomace (GP) based on a Box Behnken design was investigated using two grape varieties: Chardonnay (white variety) and Syrah (red variety). The response surface methodology was used to study the performance of the extraction conditions based on three independent variables: temperature (70 –90&#xa0;°C); time (10 –30&#xa0;min) and solvent (65% − 85% ethanol). The conditions to obtain the maximum total phenolic compounds content, as well as the antioxidant activity (assessed by the ABTS and DPPH assays) of the extracts were determined. The solvent and temperature exerted a significant effect on total phenolic content and radical scavenging activities. The optimal extraction conditions for both grape pomace varieties were: 90&#xa0;°C of temperature, 30&#xa0;min of extraction time and 65% of ethanol concentration. The grape pomace extracts corresponding to the optimal extraction conditions were analyzed using Ultra-High-Performance Liquid Chromatography-Mass Spectrometry (UHPLC-MS). UHPLC-MS analysis identified, including quinic acid, phenolic acids (gallic and protocatechuic), flavan-3-ols (catechin, epicatechin, and their derivatives) and flavonols (quercetin, kaempferol, and their derivatives), in a total of 15 and 18 compounds in Chardonnay and Syrah GP extracts, respectively. The antiproliferative WST-1 assay indicated that GP extracts had no inhibitory effect on cells viability, suggesting absence of cytotoxicity within the concentration range of 0.5–50&#xa0;µg/mL. The recovered GP extract could be used in further in vivo assays to validate its applications in food and pharmaceutical applications.</p>

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Industrial white and red grape pomace valorization: multicriteria optimization of phenolic content and antioxidant activity and assessment of the cytotoxicity of the extracts

  • 期刊论文

  • 发表日期：

    2026年5月11日

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  • Ramla Khiari,
  • Hassene Zemni,
  • Joana Madureira,
  • Susana M. Cardoso,
  • Elisabete Coelho,
  • Manuel A. Coimbra,
  • Sandra Cabo Verde,
  • Nourhene Boudhrioua

摘要

In this study, the optimization of the solid-liquid extraction of phenolic compounds from grape pomace (GP) based on a Box Behnken design was investigated using two grape varieties: Chardonnay (white variety) and Syrah (red variety). The response surface methodology was used to study the performance of the extraction conditions based on three independent variables: temperature (70 –90 °C); time (10 –30 min) and solvent (65% − 85% ethanol). The conditions to obtain the maximum total phenolic compounds content, as well as the antioxidant activity (assessed by the ABTS and DPPH assays) of the extracts were determined. The solvent and temperature exerted a significant effect on total phenolic content and radical scavenging activities. The optimal extraction conditions for both grape pomace varieties were: 90 °C of temperature, 30 min of extraction time and 65% of ethanol concentration. The grape pomace extracts corresponding to the optimal extraction conditions were analyzed using Ultra-High-Performance Liquid Chromatography-Mass Spectrometry (UHPLC-MS). UHPLC-MS analysis identified, including quinic acid, phenolic acids (gallic and protocatechuic), flavan-3-ols (catechin, epicatechin, and their derivatives) and flavonols (quercetin, kaempferol, and their derivatives), in a total of 15 and 18 compounds in Chardonnay and Syrah GP extracts, respectively. The antiproliferative WST-1 assay indicated that GP extracts had no inhibitory effect on cells viability, suggesting absence of cytotoxicity within the concentration range of 0.5–50 µg/mL. The recovered GP extract could be used in further in vivo assays to validate its applications in food and pharmaceutical applications.