<p>Perfluoroalkyl and polyfluoroalkyl substances (PFAS) are a group of emerging contaminants that have increasingly attracted research interests due to their extensive use and pervasive presence in the environment. Biochar has emerged as an inexpensive and environmental-friendly alternative for removing PFAS from water matrices due to its high sorption capacity, simplicity in design and operation, and economic viability. The abundance of biochar feedstocks further enhances the appeal of this method. This article presents a comprehensive review of studies pertaining to PFAS removal from diverse water matrices using biochar, focusing on the following key aspects: (1) efficiencies of removal of PFAS using biochar; (2) elucidation of PFAS removal mechanisms; (3) biochar feedstock and process conditions, and PFAS physico-chemical properties influencing removal mechanisms; (4) influence of water matrix properties including pH, Natural Organic Matter and ion contents, and temperature on PFAS removal efficiency; (5) assessment of reusability of biochar for PFAS treatment; and (6) challenges in scalability and economic feasibility of using biochar. A flow diagram was developed to facilitate the evaluation of PFAS removal efficiency, considering biochar and PFAS physico-chemical properties as well as water matrix constituents. The review addresses current challenges, such as the lack of sufficient research on recently introduced PFAS substitutes, hybrid treatment, biochar design and economically viable, effective regeneration approaches, scaling up and deployment challenges, and environmental and ecosystem implications. Furthermore, this review recommends real-world condition testing for efficiency evaluation. The review emphasizes that the findings reported in the literature should be validated in full-scale experiments.&#xa0;</p> Graphical Abstract <p></p>