Scholarlink

<p>This study focused on enhancing the extraction of total phenolic content (TPC), ferric reducing antioxidant power (FRAP), 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity from freeze-dried <i>Aegle marmelos</i> seeds (FDAMS) and microwave-dried <i>Aegle marmelos</i> seeds (MDAMS). The optimization process employed ultrasound-assisted extraction techniques, utilizing a response surface methodology (RSM) structured with a Box-Behnken design. The factors selected for this optimization process included temperature (40–60&#xa0;°C), time (30–60&#xa0;min), and ethanol concentration (40–80%) for both FDAMS and MDAMS. A quadratic model was established through fifteen experimental runs, and analysis of variance (ANOVA) was performed to predict the responses and validate the model’s accuracy. The proximate analysis of FDAMS and MDAMS was conducted, revealing variations in constituent components depending on the drying technique. For FDAMS, the optimal conditions of 59&#xa0;°C, 60&#xa0;min, and 51.15% ethanol concentration yielded experimental values of 99.36 ± 5.05&#xa0;mg GAE/100&#xa0;g for TPC, 3097.02 ± 52.67&#xa0;mg AAE/100&#xa0;g for FRAP, and 59.12 ± 3.67% for DPPH. Furthermore, the optimal parameters for the extraction process of MDAMS were 56.9&#xa0;°C, 60&#xa0;min, and 79% ethanol concentration. Under these optimized conditions, the empirical results were documented as 100.36 ± 4.67&#xa0;mg GAE/100&#xa0;g for TPC, 2891.46 ± 61.78&#xa0;mg AAE/100&#xa0;g for FRAP, and 51.36 ± 2.77% for DPPH. All values aligned with the predicted results, as the residual standard error was less than 0.5%.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Optimization of phenolic content and antioxidant activity from freeze- and microwave-dried Aegle marmelos seeds using ultrasound-assisted extraction and response surface methodology

  • 期刊论文

  • 发表日期：

    2026年1月27日

查看全文
  • Robin Sarkar,
  • Tanvir Ahmed,
  • Waziur Rahman Chy,
  • Md Rahmatuzzaman Rana,
  • Shuvojit Roy,
  • Razia Sultana Chowdhury

摘要

This study focused on enhancing the extraction of total phenolic content (TPC), ferric reducing antioxidant power (FRAP), 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity from freeze-dried Aegle marmelos seeds (FDAMS) and microwave-dried Aegle marmelos seeds (MDAMS). The optimization process employed ultrasound-assisted extraction techniques, utilizing a response surface methodology (RSM) structured with a Box-Behnken design. The factors selected for this optimization process included temperature (40–60 °C), time (30–60 min), and ethanol concentration (40–80%) for both FDAMS and MDAMS. A quadratic model was established through fifteen experimental runs, and analysis of variance (ANOVA) was performed to predict the responses and validate the model’s accuracy. The proximate analysis of FDAMS and MDAMS was conducted, revealing variations in constituent components depending on the drying technique. For FDAMS, the optimal conditions of 59 °C, 60 min, and 51.15% ethanol concentration yielded experimental values of 99.36 ± 5.05 mg GAE/100 g for TPC, 3097.02 ± 52.67 mg AAE/100 g for FRAP, and 59.12 ± 3.67% for DPPH. Furthermore, the optimal parameters for the extraction process of MDAMS were 56.9 °C, 60 min, and 79% ethanol concentration. Under these optimized conditions, the empirical results were documented as 100.36 ± 4.67 mg GAE/100 g for TPC, 2891.46 ± 61.78 mg AAE/100 g for FRAP, and 51.36 ± 2.77% for DPPH. All values aligned with the predicted results, as the residual standard error was less than 0.5%.