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<p> Tequila agave leaves (TAL) are residues from the tequila industry left in the fields. They pose a serious environmental threat, as a source of stenches and pest infestations, since approximately 700,000 tons are generated annually. This study proposes the integral use of TAL to produce succinic acid, since TAL contain juice, rich in soluble carbohydrates, and fibers that can be hydrolyzed. The juice was extracted from the fibers and its chemical composition was determined. Juice, fibers and delignified fibers were subjected to different hydrolysis treatments to obtain fermentable sugars: acid (H<sub>2</sub>SO<sub>4</sub> or HCl), enzymatic (Macerex<sup>R</sup> or Celuzyme<sup>R</sup>), and combined acid-enzymatic. The treatments that resulted in a higher sugar concentration were selected for succinic acid production with <i>Actinobacillus succinogenes.</i> It was found that the juice had a sugar content (glucose and fructose) of 31.3&#xa0;g/L. The fibers, on the other hand, were composed primarily of cellulose and hemicellulose (70 wt%) and the highest concentration of fermentable sugars (28.4&#xa0;g/L; 42% conversion efficiency) was achieved with fibers HCl-Celuzyme hydrolysis. Fermentations with <i>A. succinogenes</i> were carried out using the raw juice and fiber hydrolyzates (acid, enzymatic, and HCl-Celuzyme). The highest production of succinic acid was obtained with the juice (12.5&#xa0;g/L succinic acid, Y<sub>S/M</sub> = 0.48&#xa0;g/g, r<sub>S</sub> = 0.70&#xa0;g/L·h) and with HCl – Celuzyme hydrolyzates (13.6&#xa0;g/L succinic acid, Y<sub>S/M</sub> = 0.57&#xa0;g/g, r<sub>S</sub> = 0.60&#xa0;g/L·h). This study demonstrates that the leaves from tequila agave represent a promising renewable source to obtain substrates to produce succinic acid by <i>A. succinogenes.</i></p>

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Valorization of tequila agave leaves for succinic acid production by Actinobacillus succinogenes

  • 期刊论文

  • 发表日期：

    2026年1月23日

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  • David Antonio Flores-Méndez,
  • Guadalupe María Guatemala-Morales,
  • Angélica Vallejo-Giraldo,
  • Guillermo Toriz,
  • Guillermo Gosset,
  • Rosa Isela Corona-González

摘要

Tequila agave leaves (TAL) are residues from the tequila industry left in the fields. They pose a serious environmental threat, as a source of stenches and pest infestations, since approximately 700,000 tons are generated annually. This study proposes the integral use of TAL to produce succinic acid, since TAL contain juice, rich in soluble carbohydrates, and fibers that can be hydrolyzed. The juice was extracted from the fibers and its chemical composition was determined. Juice, fibers and delignified fibers were subjected to different hydrolysis treatments to obtain fermentable sugars: acid (H2SO4 or HCl), enzymatic (MacerexR or CeluzymeR), and combined acid-enzymatic. The treatments that resulted in a higher sugar concentration were selected for succinic acid production with Actinobacillus succinogenes. It was found that the juice had a sugar content (glucose and fructose) of 31.3 g/L. The fibers, on the other hand, were composed primarily of cellulose and hemicellulose (70 wt%) and the highest concentration of fermentable sugars (28.4 g/L; 42% conversion efficiency) was achieved with fibers HCl-Celuzyme hydrolysis. Fermentations with A. succinogenes were carried out using the raw juice and fiber hydrolyzates (acid, enzymatic, and HCl-Celuzyme). The highest production of succinic acid was obtained with the juice (12.5 g/L succinic acid, YS/M = 0.48 g/g, rS = 0.70 g/L·h) and with HCl – Celuzyme hydrolyzates (13.6 g/L succinic acid, YS/M = 0.57 g/g, rS = 0.60 g/L·h). This study demonstrates that the leaves from tequila agave represent a promising renewable source to obtain substrates to produce succinic acid by A. succinogenes.