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<p>Pectinase is essential in various industrial applications, such as food processing, textile manufacturing, and paper biodeinking. Nevertheless, enhancing its production poses a difficulty owing to the impact of many bioprocess variables. This research utilizes Taguchi statistical method to optimize pectinase synthesis by <i>Aspergillus niger</i> KW02, employing orange peel as a substrate in submerged fermentation, and the resulting pectinase was evaluated for biodeinking potential and green synthesis of selenium nanoparticles (SeNPs). The SeNPs were characterized using UV-Vis spectrophotometry, Fourier transform infrared spectroscopy, and transmission electron microscopy, and subsequently evaluated for biomedical applications. The L9 orthogonal array of Taguchi optimization improved pectinase yield by 4.35-fold culminating in 890.60 U/ml. The crude pectinase attained ink removal effectiveness of 83.80%, and also facilitated the green synthesis of PFN-SeNPs. The colloidal SeNPs was orange hue and spherical-shaped with λ<sub>max</sub> of 264&#xa0;nm and sizes of 62.09–64.76&#xa0;nm. Until now, there is paucity of information on nanoparticles synthesis using pectinase. The synthesized PFN-SeNPs showed substantial antibacterial efficacy against <i>Salmonella enterica</i> and <i>Klebsiella oxytoca</i> (20.10–20.80&#xa0;mm), and total fungal suppression against <i>Aspergillus flavus</i>,<i> Aspergillus niger</i>, and <i>Penicillium</i> sp. Additionally, the nanoparticles scavenged DPPH by 52.30% and displayed 100% anticoagulant activity. These findings demonstrated a cost-effective and sustainable method for pectinase and selenium nanoparticles production for sustainable development. Pectinase-inspired synthesis of nanoparticles adds to the advances in enzyme nanobiotechnology.</p> Graphical abstract <p></p>

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Enhancing pectinase production by Aspergillus niger in orange-based medium: mechanistic insights of optimization, and biotechnological applications for eco-friendly biodeinking and fabrication of selenium nanoparticles

  • 期刊论文

  • 发表日期：

    2026年1月23日

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  • S.A Abdulmumini,
  • Agbaje Lateef,
  • E.B Gueguim-Kana,
  • L.S Beukes,
  • N. Matyumza

摘要

Pectinase is essential in various industrial applications, such as food processing, textile manufacturing, and paper biodeinking. Nevertheless, enhancing its production poses a difficulty owing to the impact of many bioprocess variables. This research utilizes Taguchi statistical method to optimize pectinase synthesis by Aspergillus niger KW02, employing orange peel as a substrate in submerged fermentation, and the resulting pectinase was evaluated for biodeinking potential and green synthesis of selenium nanoparticles (SeNPs). The SeNPs were characterized using UV-Vis spectrophotometry, Fourier transform infrared spectroscopy, and transmission electron microscopy, and subsequently evaluated for biomedical applications. The L9 orthogonal array of Taguchi optimization improved pectinase yield by 4.35-fold culminating in 890.60 U/ml. The crude pectinase attained ink removal effectiveness of 83.80%, and also facilitated the green synthesis of PFN-SeNPs. The colloidal SeNPs was orange hue and spherical-shaped with λmax of 264 nm and sizes of 62.09–64.76 nm. Until now, there is paucity of information on nanoparticles synthesis using pectinase. The synthesized PFN-SeNPs showed substantial antibacterial efficacy against Salmonella enterica and Klebsiella oxytoca (20.10–20.80 mm), and total fungal suppression against Aspergillus flavus, Aspergillus niger, and Penicillium sp. Additionally, the nanoparticles scavenged DPPH by 52.30% and displayed 100% anticoagulant activity. These findings demonstrated a cost-effective and sustainable method for pectinase and selenium nanoparticles production for sustainable development. Pectinase-inspired synthesis of nanoparticles adds to the advances in enzyme nanobiotechnology.

Graphical abstract