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<p>In this study, we have synthesized zinc oxide nanoparticles (ZnO-NPs) as well as their biological applications. Further, zinc oxide <i>Bauhinia variegata</i> nanoparticles (ZnBV-NPs) were prepared by green synthesis process from zinc acetate solution through the different concentrations of <i>Bauhinia variegata</i> leaf extract. PXRD and UV–vis spectroscopy confirmed the formation of nanoparticles, and EDX confirmed the elemental composition of ZnBV-NPs, with the major elements being zinc and oxygen, while FTIR indicated the functional groups. For antibacterial studies, three bacterial strains (<i>Escherichia coli</i>, <i>Pseudomonas aeruginosa</i>, and <i>Kl</i><i>ebsiella pneumoniae</i>) were used by varying concentrations of synthesized ZnO-NPs and ZnBV-NPs which showed excellent activities against these bacterial strains. Further, 1% ZnBV-NPs-loaded carbopol was prepared for wound healing study, and for this purpose, three groups of rats were formed; in each group, six rats were placed, and group 1 was left untreated, group 2 was treated with standard quench cream, and group 3 was dressed with 1% ZnBV-NPs-loaded carbopol gel. The wound contraction of these rats was calculated at the 3rd, 5th, 7th and 9th day. One percent ZnBV-NPs-loaded carbopol gel exhibits the best wound healing activity as compared to others. DPPH and ferric ion reducing power methods were applied for scavenging activity having ascorbic acid as standard. The antioxidant power increased with increasing the concentration of NPs; ZnO-NPs concentration of 500&#xa0;µg/ml showed the highest antioxidant activity. For in vitro cell cytotoxicity testing, MTT assay was performed, and the effectiveness of ZnBV-NPs was tested against HepG2 hepatocellular cancer cells which showed no significant results.</p> Graphical Abstract <p></p>

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Green synthesis of zinc oxide nanoparticles using Bauhinia variegata leaf extract: antibacterial, antioxidant, and wound healing applications

  • 期刊论文

  • 发表日期：

    2025年4月29日

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  • Raheela Waheed,
  • Tooba Hussain,
  • Khalil Ahmad,
  • Zarmina Rashid,
  • Hina Raza,
  • Irfan Ahmad,
  • Farah Deeba,
  • Iqra Arooj

摘要

In this study, we have synthesized zinc oxide nanoparticles (ZnO-NPs) as well as their biological applications. Further, zinc oxide Bauhinia variegata nanoparticles (ZnBV-NPs) were prepared by green synthesis process from zinc acetate solution through the different concentrations of Bauhinia variegata leaf extract. PXRD and UV–vis spectroscopy confirmed the formation of nanoparticles, and EDX confirmed the elemental composition of ZnBV-NPs, with the major elements being zinc and oxygen, while FTIR indicated the functional groups. For antibacterial studies, three bacterial strains (Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumoniae) were used by varying concentrations of synthesized ZnO-NPs and ZnBV-NPs which showed excellent activities against these bacterial strains. Further, 1% ZnBV-NPs-loaded carbopol was prepared for wound healing study, and for this purpose, three groups of rats were formed; in each group, six rats were placed, and group 1 was left untreated, group 2 was treated with standard quench cream, and group 3 was dressed with 1% ZnBV-NPs-loaded carbopol gel. The wound contraction of these rats was calculated at the 3rd, 5th, 7th and 9th day. One percent ZnBV-NPs-loaded carbopol gel exhibits the best wound healing activity as compared to others. DPPH and ferric ion reducing power methods were applied for scavenging activity having ascorbic acid as standard. The antioxidant power increased with increasing the concentration of NPs; ZnO-NPs concentration of 500 µg/ml showed the highest antioxidant activity. For in vitro cell cytotoxicity testing, MTT assay was performed, and the effectiveness of ZnBV-NPs was tested against HepG2 hepatocellular cancer cells which showed no significant results.

Graphical Abstract