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<p>This study examined the impact of mixing cow manure (CM) and rumen fluid (RF) in various proportions on methane production and microbial dynamics in batch-operated digesters at three different temperatures. The proportions of RF added to CM were 0%, 10%, 20%, 30%, 40%, 50%, and 100%. The mixed substrates in triplicate bottles were then divided into three groups and incubated at temperatures of 36&#xa0;°C, 41&#xa0;°C, and 43&#xa0;°C each lasting 40&#xa0;days. In digesters operated at 36&#xa0;°C and 41&#xa0;°C, the highest methane yields produced were 292&#xa0;mL CH<sub>4</sub>/g VS<sub>added</sub> with 20% RF and 304&#xa0;mL CH<sub>4</sub>/g VS<sub>added</sub> with 40% RF, respectively, whereas that of was 189&#xa0;mL CH<sub>4</sub>/g VS<sub>added</sub> with 40% RF at 43&#xa0;°C. The digesters achieving the highest methane yields across all temperature conditions were those inoculated with RF at ratios between 20 and 40%. These digesters demonstrated the highest methane yield increases of 129%, 95%, and 121% at 36&#xa0;°C, 41&#xa0;°C, and 43&#xa0;°C, respectively, compared to the control digesters containing only CM. These findings underscore the substantial role of RF addition in enhancing biomethane production efficiency. Metagenomic analyses using next-generation sequencing (NGS) on the Ion PGM™ platform identified <i>Firmicutes, Bacteroidetes,</i> and <i>Proteobacteria</i> as the dominant bacterial groups. Quantitative polymerase chain reaction (qPCR) revealed that <i>Ruminococcus albus</i>, <i>Fibrobacter succinogenes</i>, and <i>Ruminococcus flavefaciens</i> were the main cellulose-digesting bacteria at all temperatures studied. However, the numbers of microorganisms were much lower all through the digesters operated at 43&#xa0;°C, indicating that the temperature is not suitable for microbial growth.</p> Graphical Abstract <p></p>

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Enhancing biomethane production from cattle manure by integrating rumen bacteria: a microbial analyses with next-generation sequencing and quantitative PCR

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年3月25日

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  • Gülşah Günel,
  • Orhan İnce,
  • Ömer Uzun,
  • Elif Irmak Erdem,
  • Bahar İnce

摘要

This study examined the impact of mixing cow manure (CM) and rumen fluid (RF) in various proportions on methane production and microbial dynamics in batch-operated digesters at three different temperatures. The proportions of RF added to CM were 0%, 10%, 20%, 30%, 40%, 50%, and 100%. The mixed substrates in triplicate bottles were then divided into three groups and incubated at temperatures of 36 °C, 41 °C, and 43 °C each lasting 40 days. In digesters operated at 36 °C and 41 °C, the highest methane yields produced were 292 mL CH4/g VSadded with 20% RF and 304 mL CH4/g VSadded with 40% RF, respectively, whereas that of was 189 mL CH4/g VSadded with 40% RF at 43 °C. The digesters achieving the highest methane yields across all temperature conditions were those inoculated with RF at ratios between 20 and 40%. These digesters demonstrated the highest methane yield increases of 129%, 95%, and 121% at 36 °C, 41 °C, and 43 °C, respectively, compared to the control digesters containing only CM. These findings underscore the substantial role of RF addition in enhancing biomethane production efficiency. Metagenomic analyses using next-generation sequencing (NGS) on the Ion PGM™ platform identified Firmicutes, Bacteroidetes, and Proteobacteria as the dominant bacterial groups. Quantitative polymerase chain reaction (qPCR) revealed that Ruminococcus albus, Fibrobacter succinogenes, and Ruminococcus flavefaciens were the main cellulose-digesting bacteria at all temperatures studied. However, the numbers of microorganisms were much lower all through the digesters operated at 43 °C, indicating that the temperature is not suitable for microbial growth.

Graphical Abstract