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<p>Efficient lignocellulose degradation in the environment occurs through synergistic interactions between multiple biocatalytic systems. This study aims to utilize the synergy between the ligninases and cellulases of a bacterial co-culture for the saccharification of un/pretreated rice straw. A lignocellulolytic bacterial consortium, comprising equal proportions of the cellulolytic bacteria <i>Parageobacillus thermoglucosidasius</i> NBCB1 and <i>Aeribacillus composti</i> XLN1 and the ligninolytic bacteria <i>Micrococcus yunnanensis</i> B4, showed increased cellulase production with a degree of synergism ~ 1.26, which was further augmented to ~ 1.48 upon optimization of cultural conditions through Response Surface Methodology. The triculture gave the highest cellulase production (115.68 ± 0.01&#xa0;IU/mg) in minimal salt medium containing 3% rice straw, 0.56% D-sorbitol, 0.75% peptone, medium pH 6.1, upon incubation of 2.92&#xa0;days. The lignocellulolytic biocatalysts derived from the consortium cultured in statistically optimized broth saccharified untreated, alkali- and peroxyacetic acid-pretreated rice straw with saccharification yields of ~ 82, ~ 170, and ~ 156&#xa0;mg/g rice straw, which were ~ 137, ~ 53, and ~ 58% of the yields from commercial cellulase, respectively. A synergy existed between the lignocellulolytic enzymes in the consortium, which gave significantly high saccharification yields from un/pretreated rice straw. Alkali-pretreated and untreated rice straw gave ~ 86.73 and ~ 170.83% of the reported saccharification yields from un-/similarly pretreated rice straw saccharifed by consortia, while peroxyacetic acid-pretreated rice straw gave ~ 43.33% of the reported yield from similarly pretreated rice straw saccharifed by commercial enzymes. The thermotolerant nature of the enzymes along with the effective degradation of un/pretreated rice straw established the potential of the lignocellulolytic bacterial consortium for industrial applications.</p> Graphical Abstract <p></p>

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Enzymatic synergy in lignocellulase cocktail of a thermotolerant bacterial consortium enhances rice straw saccharification

  • 期刊论文

  • 发表日期：

    2025年5月9日

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  • Arijita Basak,
  • Ayan Kumar Mahanty,
  • Shilpi Ghosh

摘要

Efficient lignocellulose degradation in the environment occurs through synergistic interactions between multiple biocatalytic systems. This study aims to utilize the synergy between the ligninases and cellulases of a bacterial co-culture for the saccharification of un/pretreated rice straw. A lignocellulolytic bacterial consortium, comprising equal proportions of the cellulolytic bacteria Parageobacillus thermoglucosidasius NBCB1 and Aeribacillus composti XLN1 and the ligninolytic bacteria Micrococcus yunnanensis B4, showed increased cellulase production with a degree of synergism ~ 1.26, which was further augmented to ~ 1.48 upon optimization of cultural conditions through Response Surface Methodology. The triculture gave the highest cellulase production (115.68 ± 0.01 IU/mg) in minimal salt medium containing 3% rice straw, 0.56% D-sorbitol, 0.75% peptone, medium pH 6.1, upon incubation of 2.92 days. The lignocellulolytic biocatalysts derived from the consortium cultured in statistically optimized broth saccharified untreated, alkali- and peroxyacetic acid-pretreated rice straw with saccharification yields of ~ 82, ~ 170, and ~ 156 mg/g rice straw, which were ~ 137, ~ 53, and ~ 58% of the yields from commercial cellulase, respectively. A synergy existed between the lignocellulolytic enzymes in the consortium, which gave significantly high saccharification yields from un/pretreated rice straw. Alkali-pretreated and untreated rice straw gave ~ 86.73 and ~ 170.83% of the reported saccharification yields from un-/similarly pretreated rice straw saccharifed by consortia, while peroxyacetic acid-pretreated rice straw gave ~ 43.33% of the reported yield from similarly pretreated rice straw saccharifed by commercial enzymes. The thermotolerant nature of the enzymes along with the effective degradation of un/pretreated rice straw established the potential of the lignocellulolytic bacterial consortium for industrial applications.

Graphical Abstract