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<p>Dark fermentation (DF) of food waste (FW) is a promising and sustainable alternative for valorization, where a carbohydrate-rich substrate is converted into value-added products such as hydrogen (H<sub>2</sub>) and volatile fatty acids (VFA). In this work, several combinations of hydrogen-producing bacteria (<i>Clostridium butyricum</i> and<i> Clostridium beijerinckii</i>) along with lactic acid bacteria (<i>Lactobacillus plantarum</i> and <i>Lactobacillus pentosus</i>) were evaluated for H<sub>2</sub> production using sterile FW as a substrate in DF batch tests as follows: 10%<sub>v/v</sub> inoculum ratio, 10 g<sub>VS</sub> L<sup>−1</sup> of FW, phosphate buffer pH 7.4 (0.005&#xa0;M), 37&#xa0;°C, and 150&#xa0;rpm. The highest H<sub>2</sub> production of 46.0 ± 0.7&#xa0;mL H<sub>2</sub> g<sub>VS</sub><sup>−1</sup> was obtained by the combination of <i>C. butyricum</i> (61%), <i>C. beijerinckii</i> (13%), <i>L. plantarum</i> (13%), and <i>L. pentosus</i> (13%), showing a synergistic activity between those strains. Subsequently, this combination was tested to carry on a bioaugmentation strategy using sterile and non-sterile FW as substrate, achieving an H<sub>2</sub> production of 89.6 ± 1.0&#xa0;mL H<sub>2</sub> g<sub>VS</sub><sup>–1</sup> and 76.7 ± 2.6&#xa0;mL H<sub>2</sub> g<sub>VS</sub><sup>–1</sup> with sterile and non-sterile FW, respectively. According to the microbial analysis, <i>Lactobacillus</i> species were displaced in the microbial community, and <i>Clostridium </i>sensu stricto<i> 1_butyricum</i> led the H<sub>2</sub> production in both sterile and non-sterile FW tests. The results suggest that a negative interaction was established between <i>C. butyricum</i> and <i>Enterobacter</i>, generating low H<sub>2</sub> production during fermentation with non-sterile FW. This study confirms that bioaugmentation with hydrogen-producing microorganisms is a promising alternative for valorizing feedstocks, such as FW, which tend to be predominated by lactic acid bacteria.</p>

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Enhanced hydrogen production from food waste via bioaugmentation with Clostridium and Lactobacillus

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年2月15日

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  • M. P. Díez,
  • E. Villanueva-Galindo,
  • I. Moreno-Andrade,
  • E. Díaz,
  • M. A. de la Rubia,
  • A. F. Mohedano,
  • M. Perez-Rangel

摘要

Dark fermentation (DF) of food waste (FW) is a promising and sustainable alternative for valorization, where a carbohydrate-rich substrate is converted into value-added products such as hydrogen (H2) and volatile fatty acids (VFA). In this work, several combinations of hydrogen-producing bacteria (Clostridium butyricum and Clostridium beijerinckii) along with lactic acid bacteria (Lactobacillus plantarum and Lactobacillus pentosus) were evaluated for H2 production using sterile FW as a substrate in DF batch tests as follows: 10%v/v inoculum ratio, 10 gVS L−1 of FW, phosphate buffer pH 7.4 (0.005 M), 37 °C, and 150 rpm. The highest H2 production of 46.0 ± 0.7 mL H2 gVS−1 was obtained by the combination of C. butyricum (61%), C. beijerinckii (13%), L. plantarum (13%), and L. pentosus (13%), showing a synergistic activity between those strains. Subsequently, this combination was tested to carry on a bioaugmentation strategy using sterile and non-sterile FW as substrate, achieving an H2 production of 89.6 ± 1.0 mL H2 gVS–1 and 76.7 ± 2.6 mL H2 gVS–1 with sterile and non-sterile FW, respectively. According to the microbial analysis, Lactobacillus species were displaced in the microbial community, and Clostridium sensu stricto 1_butyricum led the H2 production in both sterile and non-sterile FW tests. The results suggest that a negative interaction was established between C. butyricum and Enterobacter, generating low H2 production during fermentation with non-sterile FW. This study confirms that bioaugmentation with hydrogen-producing microorganisms is a promising alternative for valorizing feedstocks, such as FW, which tend to be predominated by lactic acid bacteria.