Biofabricated nanomaterials derived from Pleurotus ostreatus: novel approaches for Aedes aegypti management

Mosquito-borne diseases, such as dengue fever, Zika virus, and chikungunya, pose significant health threats globally, with Aedes aegypti being a primary vector. Despite considerable efforts to control these vectors, the emergence of insecticide resistance and environmental concerns have propelled the exploration of alternative control methods. Nanoparticles derived from natural sources have garnered attention due to their eco-friendly nature and potential efficacy against vectors. This study aimed to synthesize nanoparticles from mushroom extracts and evaluate their larvicidal activity against Aedes aegypti larvae. Mushroom extracts were prepared using a green synthesis method, followed by characterization using various techniques. The synthesized nanoparticles exhibited distinct physicochemical properties, including size, morphology, and surface chemistry. Subsequently, the larvicidal activity of the nanoparticles was assessed through bioassays conducted according to standard protocols. The larvicidal activity was dose-dependent, with higher concentrations of nanoparticles leading to increased mortality rates among larvae. Moreover, the nanoparticles exhibited minimal toxicity against non-beneficial organisms, suggesting their potential as eco-friendly larvicidal agents.