This paper addresses robust combined frequency and voltage regulation in a highly nonlinear, amphibious (aquatic and terrestrial), two-area hybrid power system (AHPS). The AHPS comprises multiple sources—thermal, dish–Stirling solar thermal (DSTS), hydro, pumped hydro, and gravity hydro units—connected to a 50-Hz grid. During operation, solar radiation fluctuations and load changes lead to significant variations in key model parameters \(({e.g., K}_{p},{T}_{p},{T}_{sc},{T}_{rh},{T}_{w},{T}_{1},{T}_{2})\) , which can cause undesirable frequency and voltage deviations. To tackle this challenge, a dynamic parameter-based modelling framework is developed for the AHPS, and a new fractional-order tilt-integral sliding mode controller (FOTI-SMC) is proposed for coordinated frequency–voltage regulation. The proposed FOTI-SMC is systematically compared with five benchmark controllers—classical PID, fractional TID, FOPID, FOTID, and nonlinear FOPISMC—whose parameters are tuned using five optimization classes (evolutionary, swarm-based, physics-based, human-inspired) and the bio-inspired Tasmanian Devil Optimization (TDO) algorithm. The TDO-tuned FOTI-SMC achieves a peak overshoot of only 1.18 for frequency deviation in area-1 (∆f1), significantly outperforming the GA-tuned PID. The proposed scheme also realizes faster recovery, with settling times reduced to 15.787s for ∆f1 and 12.052s for ∆f2, voltage deviations (∆V1 & ∆V2), and tie-line power (∆Ptie). Under realistic operating scenarios involving simultaneous variations in load, solar insolation, and modelling parameters, the TDO-FOTI-SMC maintains superior robustness, restricting overshoots to 1.517 for ∆f1 and 0.931 for ∆f2. Real-time validation on an OPAL-RT OP4510 platform confirms that the proposed TDO-optimized FOTI-SMC offers the most effective frequency–voltage regulation and disturbance rejection among all tested configurations.