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<p>Human dermal fibroblasts (HDFs) play a vital role in skin regeneration, wound healing, and tissue remodeling, making their mechanical characterization essential for understanding skin physiology and pathology. This study investigates the elastic properties of primary HDFs under physiological conditions using atomic force microscopy (AFM) and analytical contact models. The Johnson–Kendall–Roberts (JKR) model and logarithmic friction analysis were applied to quantify cell stiffness and surface interactions. Experimental force–indentation data were compared with simulation results to determine the effective Young’s modulus, which ranged between 4 and 6 kPa. The findings align with previous reports and highlight the influence of extracellular conditions on fibroblast elasticity. This work enhances the understanding of skin cell biomechanics and provides a reliable mechanical reference for future studies in tissue engineering, disease diagnosis, and regenerative medicine.</p>

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Elasticity of Dermal Fibroblasts: Insights Using AFM Contact and Friction Models

  • 期刊论文

  • 发表日期：

    2026年4月23日

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  • Zahra Sadat Eghdami,
  • Moein Taheri,
  • Ahmad Homayooni,
  • Ali Jabbari

摘要

Human dermal fibroblasts (HDFs) play a vital role in skin regeneration, wound healing, and tissue remodeling, making their mechanical characterization essential for understanding skin physiology and pathology. This study investigates the elastic properties of primary HDFs under physiological conditions using atomic force microscopy (AFM) and analytical contact models. The Johnson–Kendall–Roberts (JKR) model and logarithmic friction analysis were applied to quantify cell stiffness and surface interactions. Experimental force–indentation data were compared with simulation results to determine the effective Young’s modulus, which ranged between 4 and 6 kPa. The findings align with previous reports and highlight the influence of extracellular conditions on fibroblast elasticity. This work enhances the understanding of skin cell biomechanics and provides a reliable mechanical reference for future studies in tissue engineering, disease diagnosis, and regenerative medicine.